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Nitric Oxide Synthase in Human Parathyroid Glands and Parathyroid Adenomas

Luts, Lena LU ; Bergenfelz, Anders LU ; Alumets, Jan LU and Sundler, Frank LU (1996) In Endocrine Pathology 7(3). p.207-213
Abstract

Nitric oxide (NO) is a novel gaseous intercellular transmitter thought to play important physiological roles in the regulation of blood flow and hormone secretion in, for example, the pituitary, the thyroid, and the endocrine pancreas. Whether nitric oxide synthase (NOS) is present in the human parathyroid glands has not yet been demonstrated. In the present study, histologically normal, but functionally suppressed human parathyroid glands and parathyroid adenomas from patients with primary hyperparathyroidism were investigated by immunocytochemistry with antibodies against neuronal NOS and by reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry. We also used H&E to identify the NOS-immunoreactive... (More)

Nitric oxide (NO) is a novel gaseous intercellular transmitter thought to play important physiological roles in the regulation of blood flow and hormone secretion in, for example, the pituitary, the thyroid, and the endocrine pancreas. Whether nitric oxide synthase (NOS) is present in the human parathyroid glands has not yet been demonstrated. In the present study, histologically normal, but functionally suppressed human parathyroid glands and parathyroid adenomas from patients with primary hyperparathyroidism were investigated by immunocytochemistry with antibodies against neuronal NOS and by reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry. We also used H&E to identify the NOS-immunoreactive cells. Immunocytochemistry demonstrated the presence of neuronal-type NOS in a subpopulation of glandular cells, identified as oxyphilic cells, in both normal parathyroid glands and adenomas. NADPH-diaphorase staining visualized NOS in the endothelium of blood vessels and in glandular cells, corresponding to those containing immunoreactive NOS. In addition, we found NADPIH-diaphorase staining in many chief cells. Our results indicate that both glandular cells and vascular endothelium in human parathyroid glands and adenomas express NOS. There is thus a morphological substrate for locally produced NO that may be involved in the regulation of parathyroid blood flow and hormone secretion.

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published
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keywords
Journal Article
in
Endocrine Pathology
volume
7
issue
3
pages
7 pages
publisher
Humana Press
external identifiers
  • scopus:15144340581
ISSN
1046-3976
DOI
10.1007/BF02739923
language
English
LU publication?
yes
id
cea81564-3180-44fb-a342-d89f9993e164
date added to LUP
2017-05-10 17:40:04
date last changed
2017-11-14 09:49:35
@article{cea81564-3180-44fb-a342-d89f9993e164,
  abstract     = {<p>Nitric oxide (NO) is a novel gaseous intercellular transmitter thought to play important physiological roles in the regulation of blood flow and hormone secretion in, for example, the pituitary, the thyroid, and the endocrine pancreas. Whether nitric oxide synthase (NOS) is present in the human parathyroid glands has not yet been demonstrated. In the present study, histologically normal, but functionally suppressed human parathyroid glands and parathyroid adenomas from patients with primary hyperparathyroidism were investigated by immunocytochemistry with antibodies against neuronal NOS and by reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry. We also used H&amp;E to identify the NOS-immunoreactive cells. Immunocytochemistry demonstrated the presence of neuronal-type NOS in a subpopulation of glandular cells, identified as oxyphilic cells, in both normal parathyroid glands and adenomas. NADPH-diaphorase staining visualized NOS in the endothelium of blood vessels and in glandular cells, corresponding to those containing immunoreactive NOS. In addition, we found NADPIH-diaphorase staining in many chief cells. Our results indicate that both glandular cells and vascular endothelium in human parathyroid glands and adenomas express NOS. There is thus a morphological substrate for locally produced NO that may be involved in the regulation of parathyroid blood flow and hormone secretion.</p>},
  author       = {Luts, Lena and Bergenfelz, Anders and Alumets, Jan and Sundler, Frank},
  issn         = {1046-3976},
  keyword      = {Journal Article},
  language     = {eng},
  number       = {3},
  pages        = {207--213},
  publisher    = {Humana Press},
  series       = {Endocrine Pathology},
  title        = {Nitric Oxide Synthase in Human Parathyroid Glands and Parathyroid Adenomas},
  url          = {http://dx.doi.org/10.1007/BF02739923},
  volume       = {7},
  year         = {1996},
}