TRPA5 encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect
Liénard, Marjorie A. LU
; Baez-Nieto, David
; Tsai, Cheng-Chia
; Valencia-Montoya, Wendy A.
LU
; Werin, Balder
LU
; Johanson, Urban
LU
; Lassance, Jean-Marc
LU
; Pan, Jen Q.
; Yu, Nanfang
and Pierce, Naomi E.
(2024)
In iScience
27(4).
- Abstract
- As ectotherms, insects need heat-sensitive receptors to monitor
environmental temperatures and facilitate thermoregulation. We show that
TRPA5, a class of ankyrin transient receptor potential (TRP)
channels absent in dipteran genomes, may function as insect heat
receptors. In the triatomine bug Rhodnius prolixus (order:
Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a
uniquely high thermosensitivity, with biophysical determinants including
a large channel activation enthalpy change (72 kcal/mol), a high
temperature coefficient (Q10 = 25), and in vitro temperature-induced currents from 53°C to 68°C (T0.5 =
58.6°C), similar to... (More) - As ectotherms, insects need heat-sensitive receptors to monitor
environmental temperatures and facilitate thermoregulation. We show that
TRPA5, a class of ankyrin transient receptor potential (TRP)
channels absent in dipteran genomes, may function as insect heat
receptors. In the triatomine bug Rhodnius prolixus (order:
Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a
uniquely high thermosensitivity, with biophysical determinants including
a large channel activation enthalpy change (72 kcal/mol), a high
temperature coefficient (Q10 = 25), and in vitro temperature-induced currents from 53°C to 68°C (T0.5 =
58.6°C), similar to noxious TRPV receptors in mammals. Monomeric and
tetrameric ion channel structure predictions show reliable parallels
with fruit fly dTRPA1, with structural uniqueness in ankyrin repeat
domains, the channel selectivity filter, and potential TRP functional
modulator regions. Overall, the finding of a member of TRPA5 as a temperature-activated receptor illustrates the diversity of insect molecular heat detectors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/cf137bdc-249b-4151-86ff-72c76aa92ee2
- author
- Liénard, Marjorie A.
LU
; Baez-Nieto, David
; Tsai, Cheng-Chia
; Valencia-Montoya, Wendy A.
LU
; Werin, Balder
LU
; Johanson, Urban
LU
; Lassance, Jean-Marc
LU
; Pan, Jen Q.
; Yu, Nanfang
and Pierce, Naomi E.
- organization
- publishing date
- 2024-04-19
- type
- Contribution to journal
- publication status
- published
- subject
- in
- iScience
- volume
- 27
- issue
- 4
- article number
- 109541
- pages
- 25 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85189677135
- pmid:38577108
- ISSN
- 2589-0042
- DOI
- 10.1016/j.isci.2024.109541
- language
- English
- LU publication?
- yes
- additional info
- © 2024 The Author(s).
- id
- cf137bdc-249b-4151-86ff-72c76aa92ee2
- date added to LUP
- 2024-04-10 08:26:16
- date last changed
- 2024-04-24 12:16:56
@article{cf137bdc-249b-4151-86ff-72c76aa92ee2,
abstract = {{As ectotherms, insects need heat-sensitive receptors to monitor <br>
environmental temperatures and facilitate thermoregulation. We show that<br>
<em>TRPA5,</em> a class of ankyrin transient receptor potential (TRP) <br>
channels absent in dipteran genomes, may function as insect heat <br>
receptors. In the triatomine bug <em>Rhodnius prolixus</em> (order: <br>
Hemiptera), a vector of Chagas disease, the channel RpTRPA5B displays a <br>
uniquely high thermosensitivity, with biophysical determinants including<br>
a large channel activation enthalpy change (72 kcal/mol), a high <br>
temperature coefficient (Q<sub>10</sub> = 25), and <em>in vitro</em> temperature-induced currents from 53°C to 68°C (T<sub>0.5</sub> =<br>
58.6°C), similar to noxious TRPV receptors in mammals. Monomeric and <br>
tetrameric ion channel structure predictions show reliable parallels <br>
with fruit fly dTRPA1, with structural uniqueness in ankyrin repeat <br>
domains, the channel selectivity filter, and potential TRP functional <br>
modulator regions. Overall, the finding of a member of <em>TRPA5</em> as a temperature-activated receptor illustrates the diversity of insect molecular heat detectors.}},
author = {{Liénard, Marjorie A. and Baez-Nieto, David and Tsai, Cheng-Chia and Valencia-Montoya, Wendy A. and Werin, Balder and Johanson, Urban and Lassance, Jean-Marc and Pan, Jen Q. and Yu, Nanfang and Pierce, Naomi E.}},
issn = {{2589-0042}},
language = {{eng}},
month = {{04}},
number = {{4}},
publisher = {{Elsevier}},
series = {{iScience}},
title = {{<i>TRPA5 </i>encodes a thermosensitive ankyrin ion channel receptor in a triatomine insect}},
url = {{http://dx.doi.org/10.1016/j.isci.2024.109541}},
doi = {{10.1016/j.isci.2024.109541}},
volume = {{27}},
year = {{2024}},
}