A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans
(2016) In FEBS Letters 590(14). p.2106-2118- Abstract
The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider... (More)
The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.
(Less)
- author
- Krishnaswamyreddy, Sumitha LU ; Bågenholm, Viktoria LU ; Pudlo, Nicholas A ; Bouraoui, Hanene ; Koropatkin, Nicole M ; Martens, Eric C and Stålbrand, Henrik LU
- organization
- publishing date
- 2016-06-11
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEBS Letters
- volume
- 590
- issue
- 14
- pages
- 13 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:27288925
- scopus:84979600169
- wos:000380761100007
- ISSN
- 1873-3468
- DOI
- 10.1002/1873-3468.12250
- language
- English
- LU publication?
- yes
- id
- d21192c5-3456-4dcb-9fbd-36a34ee875bc
- date added to LUP
- 2016-06-16 15:06:55
- date last changed
- 2024-10-04 21:30:16
@article{d21192c5-3456-4dcb-9fbd-36a34ee875bc, abstract = {{<p>The Bacova_02091 gene in the β-mannan utilisation locus of Bacteroides ovatus encodes a family GH36 α-galactosidase (BoGal36A), transcriptionally upregulated during growth on galactomannan. Characterisation of recombinant BoGal36A reveals unique properties compared to other GH36 α-galactosidases, which preferentially hydrolyse terminal α-galactose in raffinose family oligosaccharides. BoGal36A prefers hydrolysing internal galactose substitutions from intact and depolymerized galactomannan. BoGal36A efficiently releases (>90%) galactose from guar and locust bean galactomannans, resulting in precipitation of the polysaccharides. As compared to other GH36 structures, the BoGal36A 3D model displays a loop deletion, resulting in a wider active site cleft which likely can accommodate a galactose-substituted polymannose backbone. This article is protected by copyright. All rights reserved.</p>}}, author = {{Krishnaswamyreddy, Sumitha and Bågenholm, Viktoria and Pudlo, Nicholas A and Bouraoui, Hanene and Koropatkin, Nicole M and Martens, Eric C and Stålbrand, Henrik}}, issn = {{1873-3468}}, language = {{eng}}, month = {{06}}, number = {{14}}, pages = {{2106--2118}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{A β-mannan utilisation locus in Bacteroides ovatus involves a GH36 α-galactosidase active on galactomannans}}, url = {{http://dx.doi.org/10.1002/1873-3468.12250}}, doi = {{10.1002/1873-3468.12250}}, volume = {{590}}, year = {{2016}}, }