Plasma membrane proteomics
(2006) In Plant Proteomics p.186-206- Abstract
- Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma... (More)
- Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma membrane proteomic studies are compared and problems with contaminating proteins are discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/d3f91c5d-20cb-4eff-84f1-d15baefd2907
- author
- Alexandersson, Erik LU ; Gustavsson, Niklas LU ; Bernfur, Katja LU ; Kjellbom, Per LU and Larsson, Christer LU
- organization
- publishing date
- 2006
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Plant Proteomics
- series title
- Plant Proteomics
- pages
- 186 - 206
- publisher
- Springer
- external identifiers
-
- scopus:33750586269
- ISBN
- 978-3-540-72617-3
- 978-3-540-72616-6
- DOI
- 10.1007/978-3-540-72617-3_13
- language
- English
- LU publication?
- yes
- id
- d3f91c5d-20cb-4eff-84f1-d15baefd2907
- date added to LUP
- 2019-06-20 17:19:39
- date last changed
- 2024-10-02 07:23:59
@inbook{d3f91c5d-20cb-4eff-84f1-d15baefd2907,
abstract = {{Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma membrane proteomic studies are compared and problems with contaminating proteins are discussed.}},
author = {{Alexandersson, Erik and Gustavsson, Niklas and Bernfur, Katja and Kjellbom, Per and Larsson, Christer}},
booktitle = {{Plant Proteomics}},
isbn = {{978-3-540-72617-3}},
language = {{eng}},
pages = {{186--206}},
publisher = {{Springer}},
series = {{Plant Proteomics}},
title = {{Plasma membrane proteomics}},
url = {{http://dx.doi.org/10.1007/978-3-540-72617-3_13}},
doi = {{10.1007/978-3-540-72617-3_13}},
year = {{2006}},
}