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Plasma membrane proteomics

Alexandersson, Erik LU ; Gustavsson, Niklas LU ; Bernfur, Katja LU ; Kjellbom, Per LU and Larsson, Christer LU (2006) In Plant Proteomics p.186-206
Abstract
Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma... (More)
Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma membrane proteomic studies are compared and problems with contaminating proteins are discussed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Plant Proteomics
series title
Plant Proteomics
pages
186 - 206
publisher
Springer
external identifiers
  • scopus:33750586269
ISBN
978-3-540-72616-6
978-3-540-72617-3
DOI
10.1007/978-3-540-72617-3_13
language
English
LU publication?
yes
id
d3f91c5d-20cb-4eff-84f1-d15baefd2907
date added to LUP
2019-06-20 17:19:39
date last changed
2019-09-27 13:07:36
@inbook{d3f91c5d-20cb-4eff-84f1-d15baefd2907,
  abstract     = {Proteins residing in the plasma membrane have key functions in transport, signal transduction, vesicle trafficking and many other important processes. To better understand these processes it is necessary to reveal the identity of plasma membrane proteins and to monitor modifications and regulation of their expression. This chapter is an overview of the methods used in plant plasma membrane proteomic studies and the results obtained so far. It focuses on studies using mass spectrometry for identification and includes aspects of plasma membrane fractionation, extraction and washing treatments, assessment of purity, separation methods for plasma membrane proteins and choice of techniques for protein cleavage. Finally, the results of plasma membrane proteomic studies are compared and problems with contaminating proteins are discussed.},
  author       = {Alexandersson, Erik and Gustavsson, Niklas and Bernfur, Katja and Kjellbom, Per and Larsson, Christer},
  isbn         = {978-3-540-72616-6},
  language     = {eng},
  pages        = {186--206},
  publisher    = {Springer},
  series       = {Plant Proteomics},
  title        = {Plasma membrane proteomics},
  url          = {http://dx.doi.org/10.1007/978-3-540-72617-3_13},
  year         = {2006},
}