Comparison of measurements of autoantibodies to glutamic acid decarboxylase and islet antigen-2 in whole blood eluates from dried blood spots using the RSR-enzyme linked immunosorbent assay kits and in-house radioimmunoassays.
(2010) In Experimental Diabetes Research 2010.- Abstract
- To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the... (More)
- To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1645210
- author
- Persson, Anders ; Becker, Charlotte LU ; Jönsson, Ida LU ; Ramelius, Anita LU and Törn, Carina LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Experimental Diabetes Research
- volume
- 2010
- article number
- 173652
- publisher
- Hindawi Limited
- external identifiers
-
- wos:000279768100001
- pmid:20613950
- scopus:77957284615
- pmid:20613950
- ISSN
- 1687-5214
- DOI
- 10.1155/2010/173652
- language
- English
- LU publication?
- yes
- id
- d425816a-3fc0-4967-b2e4-9e4195f18fc3 (old id 1645210)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20613950?dopt=Abstract
- date added to LUP
- 2016-04-04 08:56:02
- date last changed
- 2022-01-29 07:50:27
@article{d425816a-3fc0-4967-b2e4-9e4195f18fc3, abstract = {{To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; P = .008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; P < .001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.}}, author = {{Persson, Anders and Becker, Charlotte and Jönsson, Ida and Ramelius, Anita and Törn, Carina}}, issn = {{1687-5214}}, language = {{eng}}, publisher = {{Hindawi Limited}}, series = {{Experimental Diabetes Research}}, title = {{Comparison of measurements of autoantibodies to glutamic acid decarboxylase and islet antigen-2 in whole blood eluates from dried blood spots using the RSR-enzyme linked immunosorbent assay kits and in-house radioimmunoassays.}}, url = {{https://lup.lub.lu.se/search/files/5214000/1661853.pdf}}, doi = {{10.1155/2010/173652}}, volume = {{2010}}, year = {{2010}}, }