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Affinity adhesion of carbohydrate particles and yeast cells to boronate-containing polymer brushes grafted onto siliceous supports

Ivanov, Alexander LU ; Panahi, Homayon LU ; Kuzimenkova, Marina LU ; Nilsson, Lars LU ; Bergenståhl, Björn LU ; Waqif, Husain S.; Jahanshahi, Mohsen; Galaev, Igor LU and Mattiasson, Bo LU (2006) In Chemistry: A European Journal 12(27). p.7204-7214
Abstract
Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface... (More)
Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface with adhering cells was attained in 2 min. Stained cells formed denser and more stable layers on the copolymer-grafted supports than they did on supports modified with self-assembled organosilane layers derivatized with low-molecular-weight boronate, presumably due to a higher reactivity of the grafted BCCs. Quantitative detachment of adhered particles and cells could be achieved by addition of 20 mm fructose - a strong competitor for binding to boronates - at pH 7.0-9.0. Regeneration of the grafted supports allowed several sequential adhesion and detachment cycles with stained yeast cells. Affinity adhesion of micron-sized carbohydrate particles to boronate-containing polymer brushes fixed on solid supports is discussed as a possible model system suggesting a new approach to isolation and separation of living cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oligosaccharides, chemisorption, molecular recognition, cell adhesion, boronic acid
in
Chemistry: A European Journal
volume
12
issue
27
pages
7204 - 7214
publisher
John Wiley & Sons
external identifiers
  • wos:000240858400018
  • scopus:33748990906
ISSN
1521-3765
DOI
10.1002/chem.200600342
language
English
LU publication?
yes
id
d5125fc4-a082-4510-8c70-753c2942eb1e (old id 389671)
date added to LUP
2007-08-24 09:37:46
date last changed
2019-02-20 08:28:02
@article{d5125fc4-a082-4510-8c70-753c2942eb1e,
  abstract     = {Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface with adhering cells was attained in 2 min. Stained cells formed denser and more stable layers on the copolymer-grafted supports than they did on supports modified with self-assembled organosilane layers derivatized with low-molecular-weight boronate, presumably due to a higher reactivity of the grafted BCCs. Quantitative detachment of adhered particles and cells could be achieved by addition of 20 mm fructose - a strong competitor for binding to boronates - at pH 7.0-9.0. Regeneration of the grafted supports allowed several sequential adhesion and detachment cycles with stained yeast cells. Affinity adhesion of micron-sized carbohydrate particles to boronate-containing polymer brushes fixed on solid supports is discussed as a possible model system suggesting a new approach to isolation and separation of living cells.},
  author       = {Ivanov, Alexander and Panahi, Homayon and Kuzimenkova, Marina and Nilsson, Lars and Bergenståhl, Björn and Waqif, Husain S. and Jahanshahi, Mohsen and Galaev, Igor and Mattiasson, Bo},
  issn         = {1521-3765},
  keyword      = {oligosaccharides,chemisorption,molecular recognition,cell adhesion,boronic acid},
  language     = {eng},
  number       = {27},
  pages        = {7204--7214},
  publisher    = {John Wiley & Sons},
  series       = {Chemistry: A European Journal},
  title        = {Affinity adhesion of carbohydrate particles and yeast cells to boronate-containing polymer brushes grafted onto siliceous supports},
  url          = {http://dx.doi.org/10.1002/chem.200600342},
  volume       = {12},
  year         = {2006},
}