Affinity adhesion of carbohydrate particles and yeast cells to boronate-containing polymer brushes grafted onto siliceous supports
Ivanov, Alexander LU ; Panahi, Homayon LU ; Kuzimenkova, Marina LU ; Nilsson, Lars LU ; Bergenståhl, Björn LU
; Waqif, Husain S.
; Jahanshahi, Mohsen
; Galaev, Igor
LU
and Mattiasson, Bo
LU
(2006)
In Chemistry: A European Journal
12(27).
p.7204-7214
- Abstract
- Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface... (More)
- Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface with adhering cells was attained in 2 min. Stained cells formed denser and more stable layers on the copolymer-grafted supports than they did on supports modified with self-assembled organosilane layers derivatized with low-molecular-weight boronate, presumably due to a higher reactivity of the grafted BCCs. Quantitative detachment of adhered particles and cells could be achieved by addition of 20 mm fructose - a strong competitor for binding to boronates - at pH 7.0-9.0. Regeneration of the grafted supports allowed several sequential adhesion and detachment cycles with stained yeast cells. Affinity adhesion of micron-sized carbohydrate particles to boronate-containing polymer brushes fixed on solid supports is discussed as a possible model system suggesting a new approach to isolation and separation of living cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/389671
- author
- Ivanov, Alexander
LU
; Panahi, Homayon
LU
; Kuzimenkova, Marina
LU
; Nilsson, Lars
LU
; Bergenståhl, Björn
LU
; Waqif, Husain S.
; Jahanshahi, Mohsen
; Galaev, Igor
LU
and Mattiasson, Bo
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- oligosaccharides, chemisorption, molecular recognition, cell adhesion, boronic acid
- in
- Chemistry: A European Journal
- volume
- 12
- issue
- 27
- pages
- 7204 - 7214
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000240858400018
- scopus:33748990906
- ISSN
- 1521-3765
- DOI
- 10.1002/chem.200600342
- language
- English
- LU publication?
- yes
- id
- d5125fc4-a082-4510-8c70-753c2942eb1e (old id 389671)
- date added to LUP
- 2016-04-01 17:11:41
- date last changed
- 2024-10-12 11:18:14
@article{d5125fc4-a082-4510-8c70-753c2942eb1e,
abstract = {{Cross-linked agarose particles (Sepharose CL-6B) and baker's yeast cells were found to adhere to siliceous supports end-grafted with boronate-containing copolymers (BCCs) of N,N-dimethylacrylamide at pH >= 7.5, due to boronate interactions with surface carbohydrates of the particles and the cells. These interactions were registered both on macroscopic and on molecular levels: the BCCs spontaneously adsorbed on the agarose gel at pH >= 7.5, with adsorption increasing with pH. Agarose particles and yeast cells stained with Procion Red HE-3B formed stable, monolayer-like structures at pH 8.0, whereas at pH 7.0-7.8 the structures on the copolymer-grafted supports were less stable and more random. At pH 9.0, 50% saturation of the surface with adhering cells was attained in 2 min. Stained cells formed denser and more stable layers on the copolymer-grafted supports than they did on supports modified with self-assembled organosilane layers derivatized with low-molecular-weight boronate, presumably due to a higher reactivity of the grafted BCCs. Quantitative detachment of adhered particles and cells could be achieved by addition of 20 mm fructose - a strong competitor for binding to boronates - at pH 7.0-9.0. Regeneration of the grafted supports allowed several sequential adhesion and detachment cycles with stained yeast cells. Affinity adhesion of micron-sized carbohydrate particles to boronate-containing polymer brushes fixed on solid supports is discussed as a possible model system suggesting a new approach to isolation and separation of living cells.}},
author = {{Ivanov, Alexander and Panahi, Homayon and Kuzimenkova, Marina and Nilsson, Lars and Bergenståhl, Björn and Waqif, Husain S. and Jahanshahi, Mohsen and Galaev, Igor and Mattiasson, Bo}},
issn = {{1521-3765}},
keywords = {{oligosaccharides; chemisorption; molecular recognition; cell adhesion; boronic acid}},
language = {{eng}},
number = {{27}},
pages = {{7204--7214}},
publisher = {{Wiley-Blackwell}},
series = {{Chemistry: A European Journal}},
title = {{Affinity adhesion of carbohydrate particles and yeast cells to boronate-containing polymer brushes grafted onto siliceous supports}},
url = {{http://dx.doi.org/10.1002/chem.200600342}},
doi = {{10.1002/chem.200600342}},
volume = {{12}},
year = {{2006}},
}