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Nature of glomerular capillary permeability changes following acute renal ischemia/reperfusion (I/R) injury in rats.

Rippe, Catarina LU ; Rippe, Anna LU ; Larsson, Anna; Asgeirsson, Daniel LU and Rippe, Bengt LU (2006) In American Journal of Physiology: Renal, Fluid and Electrolyte Physiology 291(6). p.1362-1368
Abstract
This study was performed to evaluate the alterations of glomerular filtration barrier characteristics following acute renal ischemia-reperfusion (I/R). Ischemia was induced in anesthetized rats by unilateral renal artery occlusion for either 20 or 60 min, followed by reperfusion during 20 or 60 min, respectively, with the contralateral kidney serving as control. Sieving coefficients (theta) were obtained by analyzing Ficoll [mol.radius (a(e)) 13-85 angstrom] in urine and plasma after 20 and 60 min I/R. Furthermore, theta for human serum albumin (HSA) was estimated using a tissue uptake technique after 20 and 60 min of I/R, while clearance of HSA compared with that for neutralized HSA (nHSA) was assessed after 20 min of I/R only. Glomerular... (More)
This study was performed to evaluate the alterations of glomerular filtration barrier characteristics following acute renal ischemia-reperfusion (I/R). Ischemia was induced in anesthetized rats by unilateral renal artery occlusion for either 20 or 60 min, followed by reperfusion during 20 or 60 min, respectively, with the contralateral kidney serving as control. Sieving coefficients (theta) were obtained by analyzing Ficoll [mol.radius (a(e)) 13-85 angstrom] in urine and plasma after 20 and 60 min I/R. Furthermore, theta for human serum albumin (HSA) was estimated using a tissue uptake technique after 20 and 60 min of I/R, while clearance of HSA compared with that for neutralized HSA (nHSA) was assessed after 20 min of I/R only. Glomerular filtration rate (GFR) was measured by [Cr-51] EDTA and inulin. I/R reduced GFR and increased theta for Ficoll molecules of a(e) > 55 angstrom and theta for albumin. theta for Ficoll vs. a(e), analysed using a two-pore model, demonstrated that, despite increases in theta, the large-pore fractional ultrafiltration coefficient (alpha(L)) was unchanged after 20 min of I/R, owing to the decline in GFR, but increased after 60 min of I/R. However, the apparent alpha(L) for albumin increased already after 20 min of I/R (P < 0.005) and the nHSA/HSA clearance ratio was slightly reduced, possibly reflecting a diminished negative charge barrier. In conclusion, after 20 min of I/R, indications of a reduced charge selectivity were noted, while after 60 min of I/R, there was mainly a reduction in size selectivity, compatible with an increased formation of large pores. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxidative stress, Ficoll, fractional clearance, albumin, anoxia
in
American Journal of Physiology: Renal, Fluid and Electrolyte Physiology
volume
291
issue
6
pages
1362 - 1368
publisher
American Physiological Society
external identifiers
  • wos:000241755600030
  • scopus:33845359555
ISSN
0363-6127
DOI
10.1152/ajprenal.00123.2006
language
English
LU publication?
yes
id
d5722eea-a4ce-41a3-b71d-de007b9baf53 (old id 159151)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16822938&dopt=Abstract
date added to LUP
2007-07-10 11:52:41
date last changed
2019-11-05 02:02:38
@article{d5722eea-a4ce-41a3-b71d-de007b9baf53,
  abstract     = {This study was performed to evaluate the alterations of glomerular filtration barrier characteristics following acute renal ischemia-reperfusion (I/R). Ischemia was induced in anesthetized rats by unilateral renal artery occlusion for either 20 or 60 min, followed by reperfusion during 20 or 60 min, respectively, with the contralateral kidney serving as control. Sieving coefficients (theta) were obtained by analyzing Ficoll [mol.radius (a(e)) 13-85 angstrom] in urine and plasma after 20 and 60 min I/R. Furthermore, theta for human serum albumin (HSA) was estimated using a tissue uptake technique after 20 and 60 min of I/R, while clearance of HSA compared with that for neutralized HSA (nHSA) was assessed after 20 min of I/R only. Glomerular filtration rate (GFR) was measured by [Cr-51] EDTA and inulin. I/R reduced GFR and increased theta for Ficoll molecules of a(e) &gt; 55 angstrom and theta for albumin. theta for Ficoll vs. a(e), analysed using a two-pore model, demonstrated that, despite increases in theta, the large-pore fractional ultrafiltration coefficient (alpha(L)) was unchanged after 20 min of I/R, owing to the decline in GFR, but increased after 60 min of I/R. However, the apparent alpha(L) for albumin increased already after 20 min of I/R (P &lt; 0.005) and the nHSA/HSA clearance ratio was slightly reduced, possibly reflecting a diminished negative charge barrier. In conclusion, after 20 min of I/R, indications of a reduced charge selectivity were noted, while after 60 min of I/R, there was mainly a reduction in size selectivity, compatible with an increased formation of large pores.},
  author       = {Rippe, Catarina and Rippe, Anna and Larsson, Anna and Asgeirsson, Daniel and Rippe, Bengt},
  issn         = {0363-6127},
  keyword      = {oxidative stress,Ficoll,fractional clearance,albumin,anoxia},
  language     = {eng},
  number       = {6},
  pages        = {1362--1368},
  publisher    = {American Physiological Society},
  series       = {American Journal of Physiology: Renal, Fluid and Electrolyte Physiology},
  title        = {Nature of glomerular capillary permeability changes following acute renal ischemia/reperfusion (I/R) injury in rats.},
  url          = {http://dx.doi.org/10.1152/ajprenal.00123.2006},
  volume       = {291},
  year         = {2006},
}