Advanced

A potentiometric enzyme electrode for monitoring in organic solvents

Miyabayashi, Akiyoshi ; Reslow, Mats ; Adlercreutz, Patrick LU and Mattiasson, Bo LU (1989) In Analytica Chimica Acta 219(C). p.27-36
Abstract

A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytica Chimica Acta
volume
219
issue
C
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:0007001638
ISSN
0003-2670
DOI
10.1016/S0003-2670(00)80330-X
language
English
LU publication?
yes
id
d78dcc02-4aa9-4fea-9da6-ebc733dfcec9
date added to LUP
2019-06-22 18:56:22
date last changed
2020-01-13 02:05:15
@article{d78dcc02-4aa9-4fea-9da6-ebc733dfcec9,
  abstract     = {<p>A potentiometric enzyme electrode is described for monitoring reactions in organic solvents. By use of an enzyme deposited on magnetic particles which are attracted to the tip of the electrode by means of a magnetic field, it is possible to produce an electrode in which the enzyme can easily be exchanged. As an example, studies of the chymotrypsin-catalyzed ester synthesis in diisopropyl ether and in toluene at varying water contents are reported. The results are consistent with those obtained from batch experiments. Operational behaviour and signal stability of the system makes this kind of potentiometric enzyme electrode attractive for monitoring bioorganic processes.</p>},
  author       = {Miyabayashi, Akiyoshi and Reslow, Mats and Adlercreutz, Patrick and Mattiasson, Bo},
  issn         = {0003-2670},
  language     = {eng},
  month        = {01},
  number       = {C},
  pages        = {27--36},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {A potentiometric enzyme electrode for monitoring in organic solvents},
  url          = {http://dx.doi.org/10.1016/S0003-2670(00)80330-X},
  doi          = {10.1016/S0003-2670(00)80330-X},
  volume       = {219},
  year         = {1989},
}