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Association of recurrent venous thromboembolism and circulating microRNAs

Wang, Xiao LU ; Sundquist, Kristina LU ; Svensson, Peter J. LU ; Rastkhani, Hamideh LU ; Palmér, Karolina LU ; Memon, Ashfaque A. LU ; Sundquist, Jan LU and Zöller, Bengt LU (2019) In Clinical Epigenetics 11(1).
Abstract

Background: Patients with unprovoked first venous thromboembolism (VTE) are at a high risk of recurrence. Although circulating microRNAs (miRNAs) have been found to be associated with VTE and are markers of hypercoagulability, this study is the first to examine whether circulating miRNAs are associated with the risk of VTE recurrence. Results: A nested case-control study design was used where plasma samples were obtained from 78 patients with unprovoked VTE from the Malmö Thrombophilia Study (MATS). A total of 39 VTE patients with recurrent VTE (cases) were matched with 39 VTE patients without recurrent VTE (controls) defined by age and sex (MATS population). Plasma levels of 179 different miRNAs were evaluated in the 78 samples (after... (More)

Background: Patients with unprovoked first venous thromboembolism (VTE) are at a high risk of recurrence. Although circulating microRNAs (miRNAs) have been found to be associated with VTE and are markers of hypercoagulability, this study is the first to examine whether circulating miRNAs are associated with the risk of VTE recurrence. Results: A nested case-control study design was used where plasma samples were obtained from 78 patients with unprovoked VTE from the Malmö Thrombophilia Study (MATS). A total of 39 VTE patients with recurrent VTE (cases) were matched with 39 VTE patients without recurrent VTE (controls) defined by age and sex (MATS population). Plasma levels of 179 different miRNAs were evaluated in the 78 samples (after anticoagulant treatment was stopped) using qPCR. A total of 110 miRNAs were detected in all samples. Among those, 12 miRNAs (miR-15b-5p, miR-106a-5p, miR-197-3p, miR-652-3p, miR-361-5p, miR-222-3p, miR-26b-5p, miR-532-5p, miR-27b-3p, miR-21-5p, miR-103a-3p, and miR-30c-5p) were found to be associated with recurrent VTE after multiple correction test and conditional logistic regression analysis. A further analysis showed that miR-15b-5p, miR-197-3p, miR-27b-3p, and miR-30c-5p exhibited a trend over time, with a larger difference in miRNA levels between cases and controls for earlier recurrence. Of these 12 miRNAs, 8 miRNAs significantly correlated with circulating transforming growth factor β1/2 (TGFβ1/2). Three of them correlated with platelet count. Conclusion: We have identified 12 plasma miRNAs that may have the potential to serve as novel, non-invasive predictive biomarkers for VTE recurrence.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarker, MicroRNA, Recurrent venous thromboembolism, Risk
in
Clinical Epigenetics
volume
11
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85061535219
ISSN
1868-7075
DOI
10.1186/s13148-019-0627-z
language
English
LU publication?
yes
id
d7be58f5-f2c1-47e7-9f0d-c5e45a1d0393
date added to LUP
2019-02-21 09:01:18
date last changed
2019-03-19 04:05:53
@article{d7be58f5-f2c1-47e7-9f0d-c5e45a1d0393,
  abstract     = {<p>Background: Patients with unprovoked first venous thromboembolism (VTE) are at a high risk of recurrence. Although circulating microRNAs (miRNAs) have been found to be associated with VTE and are markers of hypercoagulability, this study is the first to examine whether circulating miRNAs are associated with the risk of VTE recurrence. Results: A nested case-control study design was used where plasma samples were obtained from 78 patients with unprovoked VTE from the Malmö Thrombophilia Study (MATS). A total of 39 VTE patients with recurrent VTE (cases) were matched with 39 VTE patients without recurrent VTE (controls) defined by age and sex (MATS population). Plasma levels of 179 different miRNAs were evaluated in the 78 samples (after anticoagulant treatment was stopped) using qPCR. A total of 110 miRNAs were detected in all samples. Among those, 12 miRNAs (miR-15b-5p, miR-106a-5p, miR-197-3p, miR-652-3p, miR-361-5p, miR-222-3p, miR-26b-5p, miR-532-5p, miR-27b-3p, miR-21-5p, miR-103a-3p, and miR-30c-5p) were found to be associated with recurrent VTE after multiple correction test and conditional logistic regression analysis. A further analysis showed that miR-15b-5p, miR-197-3p, miR-27b-3p, and miR-30c-5p exhibited a trend over time, with a larger difference in miRNA levels between cases and controls for earlier recurrence. Of these 12 miRNAs, 8 miRNAs significantly correlated with circulating transforming growth factor β1/2 (TGFβ1/2). Three of them correlated with platelet count. Conclusion: We have identified 12 plasma miRNAs that may have the potential to serve as novel, non-invasive predictive biomarkers for VTE recurrence.</p>},
  articleno    = {28},
  author       = {Wang, Xiao and Sundquist, Kristina and Svensson, Peter J. and Rastkhani, Hamideh and Palmér, Karolina and Memon, Ashfaque A. and Sundquist, Jan and Zöller, Bengt},
  issn         = {1868-7075},
  keyword      = {Biomarker,MicroRNA,Recurrent venous thromboembolism,Risk},
  language     = {eng},
  month        = {02},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Clinical Epigenetics},
  title        = {Association of recurrent venous thromboembolism and circulating microRNAs},
  url          = {http://dx.doi.org/10.1186/s13148-019-0627-z},
  volume       = {11},
  year         = {2019},
}