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Directly Converted Human Fibroblasts Mature to Neurons and Show Long-Term Survival in Adult Rodent Hippocampus

Avaliani, Natalia LU ; Pfisterer, Ulrich LU ; Heuer, Andreas LU ; Parmar, Malin LU ; Kokaia, Merab LU and Andersson, My LU (2017) In Stem Cells International 2017.
Abstract

Direct conversion of human somatic cells to induced neurons (iNs), using lineage-specific transcription factors has opened new opportunities for cell therapy in a number of neurological diseases, including epilepsy. In most severe cases of epilepsy, seizures often originate in the hippocampus, where populations of inhibitory interneurons degenerate. Thus, iNs could be of potential use to replace these lost interneurons. It is not known, however, if iNs survive and maintain functional neuronal properties for prolonged time periods in in vivo. We transplanted human fibroblast-derived iNs into the adult rat hippocampus and observed a progressive morphological differentiation, with more developed dendritic arborisation at six months as... (More)

Direct conversion of human somatic cells to induced neurons (iNs), using lineage-specific transcription factors has opened new opportunities for cell therapy in a number of neurological diseases, including epilepsy. In most severe cases of epilepsy, seizures often originate in the hippocampus, where populations of inhibitory interneurons degenerate. Thus, iNs could be of potential use to replace these lost interneurons. It is not known, however, if iNs survive and maintain functional neuronal properties for prolonged time periods in in vivo. We transplanted human fibroblast-derived iNs into the adult rat hippocampus and observed a progressive morphological differentiation, with more developed dendritic arborisation at six months as compared to one month. This was accompanied by mature electrophysiological properties and fast high amplitude action potentials at six months after transplantation. This proof-of-principle study suggests that human iNs can be developed as a candidate source for cell replacement therapy in temporal lobe epilepsy.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
Stem Cells International
volume
2017
pages
9 pages
publisher
Hindawi Publishing Corporation
external identifiers
  • scopus:85042854268
ISSN
1687-966X
DOI
10.1155/2017/5718608
language
English
LU publication?
yes
id
d90b057b-cea4-4d63-8a23-b9e05bb070bc
date added to LUP
2018-03-15 13:58:29
date last changed
2019-04-13 02:17:02
@article{d90b057b-cea4-4d63-8a23-b9e05bb070bc,
  abstract     = {<p>Direct conversion of human somatic cells to induced neurons (iNs), using lineage-specific transcription factors has opened new opportunities for cell therapy in a number of neurological diseases, including epilepsy. In most severe cases of epilepsy, seizures often originate in the hippocampus, where populations of inhibitory interneurons degenerate. Thus, iNs could be of potential use to replace these lost interneurons. It is not known, however, if iNs survive and maintain functional neuronal properties for prolonged time periods in in vivo. We transplanted human fibroblast-derived iNs into the adult rat hippocampus and observed a progressive morphological differentiation, with more developed dendritic arborisation at six months as compared to one month. This was accompanied by mature electrophysiological properties and fast high amplitude action potentials at six months after transplantation. This proof-of-principle study suggests that human iNs can be developed as a candidate source for cell replacement therapy in temporal lobe epilepsy.</p>},
  articleno    = {5718608},
  author       = {Avaliani, Natalia and Pfisterer, Ulrich and Heuer, Andreas and Parmar, Malin and Kokaia, Merab and Andersson, My},
  issn         = {1687-966X},
  language     = {eng},
  month        = {11},
  pages        = {9},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Stem Cells International},
  title        = {Directly Converted Human Fibroblasts Mature to Neurons and Show Long-Term Survival in Adult Rodent Hippocampus},
  url          = {http://dx.doi.org/10.1155/2017/5718608},
  volume       = {2017},
  year         = {2017},
}