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Xylo- and arabinoxylooligosaccharides from wheat bran by endoxylanases, utilisation by probiotic bacteria, and structural studies of the enzymes

Mathew, Sindhu LU ; Aronsson, Anna LU ; Karlsson, Eva Nordberg LU and Adlercreutz, Patrick LU (2018) In Applied Microbiology and Biotechnology
Abstract

Xylooligosaccharides (XOS) and arabinoxylooligosaccharides (AXOS) were produced from the insoluble arabinoxylan fraction of pretreated wheat bran by endoxylanases. The glycoside hydrolase (GH) family 10 xylanases GsXyn10A from Geobacillus stearothermophilus and RmXyn10A-CM from Rhodothermus marinus produced the AXOS A3X, A2XX and A2 + 3XX in addition to XOS. RmXyn10A-CM also produced XA2 + 3XX due to its non-conserved aglycone region accommodating additional arabinose substitutions in subsite +2. The GH11 enzymes, Pentopan from Thermomyces lanuginosus and NpXyn11A from Neocallimastix patriciarum had minor structural differences affecting hydrogen bonds in subsites −3 and +3, with similar... (More)

Xylooligosaccharides (XOS) and arabinoxylooligosaccharides (AXOS) were produced from the insoluble arabinoxylan fraction of pretreated wheat bran by endoxylanases. The glycoside hydrolase (GH) family 10 xylanases GsXyn10A from Geobacillus stearothermophilus and RmXyn10A-CM from Rhodothermus marinus produced the AXOS A3X, A2XX and A2 + 3XX in addition to XOS. RmXyn10A-CM also produced XA2 + 3XX due to its non-conserved aglycone region accommodating additional arabinose substitutions in subsite +2. The GH11 enzymes, Pentopan from Thermomyces lanuginosus and NpXyn11A from Neocallimastix patriciarum had minor structural differences affecting hydrogen bonds in subsites −3 and +3, with similar hydrolysis profiles producing XA3XX as major AXOS and minor amounts of XA2XX but different ratios of X3/X2. In vitro analysis of the prebiotic properties of (A)XOS produced by Pentopan revealed nearly complete uptake of X2 and X3 by the probiotic bacteria Lactobacillus brevis and Bifidobacterium adolescentis. In contrast to previous reports, the GH43 arabinofuranosidase BaAXHd-3 from B. adolescentis cleaved α-1,3-linked arabinose on some single substituted AXOS.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Arabinofuranosidases, Endoxylanases, Probiotics, Structural bioinformatics, Xylooligosaccharides
in
Applied Microbiology and Biotechnology
pages
16 pages
publisher
Springer
external identifiers
  • scopus:85042136753
ISSN
0175-7598
DOI
10.1007/s00253-018-8823-x
language
English
LU publication?
yes
id
da325b54-58a0-427b-b3df-148c6f4ab099
date added to LUP
2018-03-06 11:01:25
date last changed
2018-05-29 11:25:08
@article{da325b54-58a0-427b-b3df-148c6f4ab099,
  abstract     = {<p>Xylooligosaccharides (XOS) and arabinoxylooligosaccharides (AXOS) were produced from the insoluble arabinoxylan fraction of pretreated wheat bran by endoxylanases. The glycoside hydrolase (GH) family 10 xylanases GsXyn10A from Geobacillus stearothermophilus and RmXyn10A-CM from Rhodothermus marinus produced the AXOS A<sup>3</sup>X, A<sup>2</sup>XX and A<sup>2 + 3</sup>XX in addition to XOS. RmXyn10A-CM also produced XA<sup>2 + 3</sup>XX due to its non-conserved aglycone region accommodating additional arabinose substitutions in subsite +2. The GH11 enzymes, Pentopan from Thermomyces lanuginosus and NpXyn11A from Neocallimastix patriciarum had minor structural differences affecting hydrogen bonds in subsites −3 and +3, with similar hydrolysis profiles producing XA<sup>3</sup>XX as major AXOS and minor amounts of XA<sup>2</sup>XX but different ratios of X<sub>3</sub>/X<sub>2</sub>. In vitro analysis of the prebiotic properties of (A)XOS produced by Pentopan revealed nearly complete uptake of X<sub>2</sub> and X<sub>3</sub> by the probiotic bacteria Lactobacillus brevis and Bifidobacterium adolescentis. In contrast to previous reports, the GH43 arabinofuranosidase BaAXHd-3 from B. adolescentis cleaved α-1,3-linked arabinose on some single substituted AXOS.</p>},
  author       = {Mathew, Sindhu and Aronsson, Anna and Karlsson, Eva Nordberg and Adlercreutz, Patrick},
  issn         = {0175-7598},
  keyword      = {Arabinofuranosidases,Endoxylanases,Probiotics,Structural bioinformatics,Xylooligosaccharides},
  language     = {eng},
  month        = {02},
  pages        = {16},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Xylo- and arabinoxylooligosaccharides from wheat bran by endoxylanases, utilisation by probiotic bacteria, and structural studies of the enzymes},
  url          = {http://dx.doi.org/10.1007/s00253-018-8823-x},
  year         = {2018},
}