Growth pattern of experimental glioblastoma
(2020) In Histology and Histopathology 35(8). p.871-886- Abstract
- Glioblastoma multiforme (GBM) is an aggressive primary brain malignancy with a very poor prognosis. Researchers employ animal models to develop potential therapies. It is important that these models have clinical relevance. This means that old models, propagated for decades in cultures, should be questioned. Parameters to be evaluated include whether animals are immune competent or not, the infiltrative growth pattern of the tumor, tumor volume resulting in symptoms and growth rate.
We here describe the growth pattern of an experimental glioblastoma model in detail with GFP positive glioblastoma cells in fully immune competent animals
and study tumor growth rate and tumor mass as a function of time from... (More) - Glioblastoma multiforme (GBM) is an aggressive primary brain malignancy with a very poor prognosis. Researchers employ animal models to develop potential therapies. It is important that these models have clinical relevance. This means that old models, propagated for decades in cultures, should be questioned. Parameters to be evaluated include whether animals are immune competent or not, the infiltrative growth pattern of the tumor, tumor volume resulting in symptoms and growth rate.
We here describe the growth pattern of an experimental glioblastoma model in detail with GFP positive glioblastoma cells in fully immune competent animals
and study tumor growth rate and tumor mass as a function of time from inoculation.
We were able to correlate findings made with classical immunohistochemistry and MR findings. The tumor growth rate was fitted by a Gompertz function. The model predicted the time until onset of symptoms for 5000 inoculated cells to 18.7±0.4 days, and the tumor mass at days 10 and 14, which are commonly used as the start of treatment in
therapeutic studies, were 5.97±0.62 mg and 29.1±3.0 mg, respectively.
We want to raise the question regarding the clinical relevance of
the outline of glioblastoma experiments, where treatment is often
initiated at a very early stage. The approach presented here
could potentially be modified to gain information also from other tumor models. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/dc8d7131-6224-48d4-b895-43013c523303
- author
- Ahlstedt, Jonatan
LU
; Förnvik, Karolina LU
; Helms, Gunther LU
; Salford, Leif G LU ; Ceberg, Crister LU
; Skagerberg, Gunnar LU and Nittby, Henrietta LU
- organization
- publishing date
- 2020-08
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Histology and Histopathology
- volume
- 35
- issue
- 8
- pages
- 871 - 886
- publisher
- Histology and Histopathology
- external identifiers
-
- pmid:32022242
- scopus:85090508420
- ISSN
- 1699-5848
- DOI
- 10.14670/HH-18-207
- project
- Kartläggning av NS1 tumörer i råttor på en klinisk 3T MR kamera
- language
- English
- LU publication?
- yes
- id
- dc8d7131-6224-48d4-b895-43013c523303
- date added to LUP
- 2020-02-17 16:40:25
- date last changed
- 2023-04-10 08:32:18
@article{dc8d7131-6224-48d4-b895-43013c523303, abstract = {{Glioblastoma multiforme (GBM) is an aggressive primary brain malignancy with a very poor prognosis. Researchers employ animal models to develop potential therapies. It is important that these models have clinical relevance. This means that old models, propagated for decades in cultures, should be questioned. Parameters to be evaluated include whether animals are immune competent or not, the infiltrative growth pattern of the tumor, tumor volume resulting in symptoms and growth rate.<br/>We here describe the growth pattern of an experimental glioblastoma model in detail with GFP positive glioblastoma cells in fully immune competent animals<br/>and study tumor growth rate and tumor mass as a function of time from inoculation.<br/>We were able to correlate findings made with classical immunohistochemistry and MR findings. The tumor growth rate was fitted by a Gompertz function. The model predicted the time until onset of symptoms for 5000 inoculated cells to 18.7±0.4 days, and the tumor mass at days 10 and 14, which are commonly used as the start of treatment in <br/>therapeutic studies, were 5.97±0.62 mg and 29.1±3.0 mg, respectively.<br/>We want to raise the question regarding the clinical relevance of <br/>the outline of glioblastoma experiments, where treatment is often<br/>initiated at a very early stage. The approach presented here <br/>could potentially be modified to gain information also from other tumor models.}}, author = {{Ahlstedt, Jonatan and Förnvik, Karolina and Helms, Gunther and Salford, Leif G and Ceberg, Crister and Skagerberg, Gunnar and Nittby, Henrietta}}, issn = {{1699-5848}}, language = {{eng}}, number = {{8}}, pages = {{871--886}}, publisher = {{Histology and Histopathology}}, series = {{Histology and Histopathology}}, title = {{Growth pattern of experimental glioblastoma}}, url = {{http://dx.doi.org/10.14670/HH-18-207}}, doi = {{10.14670/HH-18-207}}, volume = {{35}}, year = {{2020}}, }