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Arabidopsis phospholipid modifications mediate cellulase-induced resistance to a fungal peptide antibiotic by imposing cell polarity

Panthapulakkal Narayanan, Saritha LU ; Dotson, Bradley R LU orcid ; Noack, Lise ; Holla, Sanjana LU ; Ren, Shichao LU ; Dörmann, Peter ; Widell, Susanne LU ; Persson, Staffan LU ; Lager, Ida LU and Rasmusson, Allan G LU orcid (2026) In New Phytologist 249(2). p.975-991
Abstract

Plant-symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane-permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase-induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13, a phospholipase Dζ, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal... (More)

Plant-symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane-permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase-induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13, a phospholipase Dζ, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal root epidermal PMs. Consistently, the CIRA response was reversed by addition of lysoPS. CIRA13 is essential for vesicle trafficking, which in turn is crucial for CIRA induction. Overall, cellulase induces a cellular polarity with respect to phospholipids, not previously observed in plants, that is leading to increased lipid packing and preventing peptaibol permeabilization of the outer periclinal membrane.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Phytologist
volume
249
issue
2
pages
975 - 991
publisher
Wiley-Blackwell
external identifiers
  • scopus:105021315222
  • pmid:41204768
ISSN
1469-8137
DOI
10.1111/nph.70721
language
English
LU publication?
yes
additional info
© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.
id
dd14ce01-a92f-4d57-9c3a-eeeffca65ba4
date added to LUP
2025-12-01 14:27:33
date last changed
2025-12-30 06:35:50
@article{dd14ce01-a92f-4d57-9c3a-eeeffca65ba4,
  abstract     = {{<p>Plant-symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane-permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase-induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13, a phospholipase Dζ, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal root epidermal PMs. Consistently, the CIRA response was reversed by addition of lysoPS. CIRA13 is essential for vesicle trafficking, which in turn is crucial for CIRA induction. Overall, cellulase induces a cellular polarity with respect to phospholipids, not previously observed in plants, that is leading to increased lipid packing and preventing peptaibol permeabilization of the outer periclinal membrane.</p>}},
  author       = {{Panthapulakkal Narayanan, Saritha and Dotson, Bradley R and Noack, Lise and Holla, Sanjana and Ren, Shichao and Dörmann, Peter and Widell, Susanne and Persson, Staffan and Lager, Ida and Rasmusson, Allan G}},
  issn         = {{1469-8137}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{975--991}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{New Phytologist}},
  title        = {{Arabidopsis phospholipid modifications mediate cellulase-induced resistance to a fungal peptide antibiotic by imposing cell polarity}},
  url          = {{http://dx.doi.org/10.1111/nph.70721}},
  doi          = {{10.1111/nph.70721}},
  volume       = {{249}},
  year         = {{2026}},
}