ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation
(2016) In Open biology 6(4).- Abstract
In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic... (More)
In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic hyphae. We showed that sporogenic hyphae stop extending at the time of ParA accumulation and Z-ring formation. We demonstrated that both ParA and ParB affect the rate of hyphal extension. Additionally, we showed that ParA promotes the formation of massive nucleoprotein complexes by ParB.We also showed that FtsZ ring assembly is affected by the ParB protein and/or unsegregated DNA. Our results indicate the existence of a checkpoint between the extension and septation of sporogenic hyphae that involves the ParA and ParB proteins.
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- author
- Donczew, Magdalena ; Mackiewicz, Paweł ; Wróbel, Agnieszka ; Flärdh, Klas LU ; Zakrzewska-Czerwińska, Jolanta and Jakimowicz, Dagmara
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacterial cell division, Bacterial cell growth, Chromosome segregation, ParAB, Sporulation, Streptomyces
- in
- Open biology
- volume
- 6
- issue
- 4
- article number
- 150263
- pages
- 15 pages
- publisher
- Royal Society Publishing
- external identifiers
-
- scopus:85007569476
- pmid:27248800
- ISSN
- 2046-2441
- DOI
- 10.1098/rsob.150263
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2016 The Authors. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
- id
- dde8954b-7991-44c3-95e1-fcd466819b2a
- date added to LUP
- 2021-03-29 18:39:21
- date last changed
- 2024-06-16 12:02:24
@article{dde8954b-7991-44c3-95e1-fcd466819b2a,
abstract = {{<p>In unicellular bacteria, the ParA and ParB proteins segregate chromosomes and coordinate this process with cell division and chromosome replication. During sporulation of mycelial Streptomyces, ParA and ParB uniformly distribute multiple chromosomes along the filamentous sporogenic hyphal compartment, which then differentiates into a chain of unigenomic spores. However, chromosome segregation must be coordinated with cell elongation and multiple divisions. Here, we addressed the question of whether ParA and ParB are involved in the synchronization of cell-cycle processes during sporulation in Streptomyces. To answer this question, we used time-lapse microscopy, which allows the monitoring of growth and division of single sporogenic hyphae. We showed that sporogenic hyphae stop extending at the time of ParA accumulation and Z-ring formation. We demonstrated that both ParA and ParB affect the rate of hyphal extension. Additionally, we showed that ParA promotes the formation of massive nucleoprotein complexes by ParB.We also showed that FtsZ ring assembly is affected by the ParB protein and/or unsegregated DNA. Our results indicate the existence of a checkpoint between the extension and septation of sporogenic hyphae that involves the ParA and ParB proteins.</p>}},
author = {{Donczew, Magdalena and Mackiewicz, Paweł and Wróbel, Agnieszka and Flärdh, Klas and Zakrzewska-Czerwińska, Jolanta and Jakimowicz, Dagmara}},
issn = {{2046-2441}},
keywords = {{Bacterial cell division; Bacterial cell growth; Chromosome segregation; ParAB; Sporulation; Streptomyces}},
language = {{eng}},
number = {{4}},
publisher = {{Royal Society Publishing}},
series = {{Open biology}},
title = {{ParA and ParB coordinate chromosome segregation with cell elongation and division during Streptomyces sporulation}},
url = {{http://dx.doi.org/10.1098/rsob.150263}},
doi = {{10.1098/rsob.150263}},
volume = {{6}},
year = {{2016}},
}