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Lipase-catalyzed reactions in organic and supercritical solvents : Application to fat-soluble vitamin determination in milk powder and infant formula

Turner, Charlotta LU orcid ; Persson, Mattias ; Mathiasson, Lennart LU ; Adlercreutz, Patrick LU orcid and King, Jerry W. (2001) In Enzyme and Microbial Technology 29(2-3). p.111-121
Abstract

The aim of this study was to thoroughly investigate the possibility of using enzyme catalyzed hydrolysis and alcoholysis of ester bonds in vitamin A and E esters to facilitate their determination in different food formulas. Two vitamin esters, retinyl palmitate and α-tocopheryl acetate were used as model compounds and two food formulas, milk powder and infant formula, were used as model matrices. Six lipase preparations and one esterase preparation were investigated in the solvents di-isopropyl ether, hexane/ethanol and supercritical carbon dioxide containing ethanol. Three of the enzyme preparations, lipases from Candida antarctica (Novozyme 435), Rhizomucor miehei (Lipozyme IM) and Pseudomonas cepacia, showed considerably higher... (More)

The aim of this study was to thoroughly investigate the possibility of using enzyme catalyzed hydrolysis and alcoholysis of ester bonds in vitamin A and E esters to facilitate their determination in different food formulas. Two vitamin esters, retinyl palmitate and α-tocopheryl acetate were used as model compounds and two food formulas, milk powder and infant formula, were used as model matrices. Six lipase preparations and one esterase preparation were investigated in the solvents di-isopropyl ether, hexane/ethanol and supercritical carbon dioxide containing ethanol. Three of the enzyme preparations, lipases from Candida antarctica (Novozyme 435), Rhizomucor miehei (Lipozyme IM) and Pseudomonas cepacia, showed considerably higher activity toward retinyl palmitate than the other four enzyme preparations. There was no observed activity with α-tocopheryl acetate using any of the enzyme preparations. Novozyme 435 showed highest activity in supercritical fluid and generally larger tolerance to variations of the investigated parameters. Using this enzyme preparation in supercritical carbon dioxide containing 3 vol% ethanol and 0.03 vol% water at 366 bar and 80°C, quantitative conversion of retinyl palmitate to retinol was obtained. These conditions were then used for simultaneous lipase-catalyzed reaction and extraction of vitamin A and E from milk powder and infant formula. The developed supercritical fluid extraction method using immobilized Candida antarctica preparation seems to be more beneficial to the oxidation prone vitamins A and E compared to extraction methodologies based on alkaline saponification, resulting in comparatively higher recoveries.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alcoholysis, Hydrolysis, Lipase, Organic solvent, Retinyl palmitate, SFE
in
Enzyme and Microbial Technology
volume
29
issue
2-3
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:0035822749
ISSN
0141-0229
DOI
10.1016/S0141-0229(01)00359-3
language
English
LU publication?
yes
id
e07a7bf5-6296-4617-a97f-45de0f7f25e5
date added to LUP
2019-06-20 15:49:35
date last changed
2024-01-30 23:28:37
@article{e07a7bf5-6296-4617-a97f-45de0f7f25e5,
  abstract     = {{<p>The aim of this study was to thoroughly investigate the possibility of using enzyme catalyzed hydrolysis and alcoholysis of ester bonds in vitamin A and E esters to facilitate their determination in different food formulas. Two vitamin esters, retinyl palmitate and α-tocopheryl acetate were used as model compounds and two food formulas, milk powder and infant formula, were used as model matrices. Six lipase preparations and one esterase preparation were investigated in the solvents di-isopropyl ether, hexane/ethanol and supercritical carbon dioxide containing ethanol. Three of the enzyme preparations, lipases from Candida antarctica (Novozyme 435), Rhizomucor miehei (Lipozyme IM) and Pseudomonas cepacia, showed considerably higher activity toward retinyl palmitate than the other four enzyme preparations. There was no observed activity with α-tocopheryl acetate using any of the enzyme preparations. Novozyme 435 showed highest activity in supercritical fluid and generally larger tolerance to variations of the investigated parameters. Using this enzyme preparation in supercritical carbon dioxide containing 3 vol% ethanol and 0.03 vol% water at 366 bar and 80°C, quantitative conversion of retinyl palmitate to retinol was obtained. These conditions were then used for simultaneous lipase-catalyzed reaction and extraction of vitamin A and E from milk powder and infant formula. The developed supercritical fluid extraction method using immobilized Candida antarctica preparation seems to be more beneficial to the oxidation prone vitamins A and E compared to extraction methodologies based on alkaline saponification, resulting in comparatively higher recoveries.</p>}},
  author       = {{Turner, Charlotta and Persson, Mattias and Mathiasson, Lennart and Adlercreutz, Patrick and King, Jerry W.}},
  issn         = {{0141-0229}},
  keywords     = {{Alcoholysis; Hydrolysis; Lipase; Organic solvent; Retinyl palmitate; SFE}},
  language     = {{eng}},
  month        = {{08}},
  number       = {{2-3}},
  pages        = {{111--121}},
  publisher    = {{Elsevier}},
  series       = {{Enzyme and Microbial Technology}},
  title        = {{Lipase-catalyzed reactions in organic and supercritical solvents : Application to fat-soluble vitamin determination in milk powder and infant formula}},
  url          = {{http://dx.doi.org/10.1016/S0141-0229(01)00359-3}},
  doi          = {{10.1016/S0141-0229(01)00359-3}},
  volume       = {{29}},
  year         = {{2001}},
}