Advanced

Hydrolysis of maltoheptaose in flow through silicon wafer microreactors containing immobilised alpha-amylase and glycoamylase

Melander, Claes LU ; Tuting, W; Bengtsson, Martin LU ; Laurell, Thomas LU ; Mischnick, P and Gorton, Lo LU (2006) In Stärke 58(5). p.231-242
Abstract
In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be... (More)
In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be used for hydrolysis of starch and also additionally be connected directly on-line with, e.g., liquid chromatography, making it possible to perform on-line characterisation and analysis of starch hydrolysis products. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycoamylase, immobilised enzyme silicon reactor, enzymatic degradation, alpha-amylase
in
Stärke
volume
58
issue
5
pages
231 - 242
publisher
John Wiley & Sons
external identifiers
  • wos:000237790700004
  • scopus:33646739464
ISSN
0038-9056
DOI
10.1002/star.200500450
language
English
LU publication?
yes
id
e26c1227-a64b-45d8-973d-85c963f33e17 (old id 408489)
date added to LUP
2007-10-10 12:06:58
date last changed
2019-02-20 04:05:36
@article{e26c1227-a64b-45d8-973d-85c963f33e17,
  abstract     = {In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be used for hydrolysis of starch and also additionally be connected directly on-line with, e.g., liquid chromatography, making it possible to perform on-line characterisation and analysis of starch hydrolysis products.},
  author       = {Melander, Claes and Tuting, W and Bengtsson, Martin and Laurell, Thomas and Mischnick, P and Gorton, Lo},
  issn         = {0038-9056},
  keyword      = {glycoamylase,immobilised enzyme silicon reactor,enzymatic degradation,alpha-amylase},
  language     = {eng},
  number       = {5},
  pages        = {231--242},
  publisher    = {John Wiley & Sons},
  series       = {Stärke},
  title        = {Hydrolysis of maltoheptaose in flow through silicon wafer microreactors containing immobilised alpha-amylase and glycoamylase},
  url          = {http://dx.doi.org/10.1002/star.200500450},
  volume       = {58},
  year         = {2006},
}