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Hydrolysis of maltoheptaose in flow through silicon wafer microreactors containing immobilised alpha-amylase and glycoamylase

Melander, Claes LU ; Tuting, W ; Bengtsson, Martin LU ; Laurell, Thomas LU ; Mischnick, P and Gorton, Lo LU (2006) In Stärke 58(5). p.231-242
Abstract
In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be... (More)
In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be used for hydrolysis of starch and also additionally be connected directly on-line with, e.g., liquid chromatography, making it possible to perform on-line characterisation and analysis of starch hydrolysis products. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycoamylase, immobilised enzyme silicon reactor, enzymatic degradation, alpha-amylase
in
Stärke
volume
58
issue
5
pages
231 - 242
publisher
John Wiley and Sons Inc.
external identifiers
  • wos:000237790700004
  • scopus:33646739464
ISSN
0038-9056
DOI
10.1002/star.200500450
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011)
id
e26c1227-a64b-45d8-973d-85c963f33e17 (old id 408489)
date added to LUP
2016-04-01 11:59:44
date last changed
2020-03-24 02:38:14
@article{e26c1227-a64b-45d8-973d-85c963f33e17,
  abstract     = {In this study,a silicon micro immobilised enzyme reactor (mu IMER) has been applied for hydrolysis of maltoheptaose as a model maltodextrin and starch using immobilised otamylase (from Aspergillus oryzae) and glycoamylase (from Aspergillus niger). The influence of several parameters was investigated such as immobilisation chemistry, buffer constituents, pH, temperature, flow rate and substrate concentration. The conversion efficiency profile of the substrate was measured and the long-term stability of the reactor was tested. For separation and detection of the formed hydrolysis products, high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used. The results show that the mu IMERs can also be used for hydrolysis of starch and also additionally be connected directly on-line with, e.g., liquid chromatography, making it possible to perform on-line characterisation and analysis of starch hydrolysis products.},
  author       = {Melander, Claes and Tuting, W and Bengtsson, Martin and Laurell, Thomas and Mischnick, P and Gorton, Lo},
  issn         = {0038-9056},
  language     = {eng},
  number       = {5},
  pages        = {231--242},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Stärke},
  title        = {Hydrolysis of maltoheptaose in flow through silicon wafer microreactors containing immobilised alpha-amylase and glycoamylase},
  url          = {http://dx.doi.org/10.1002/star.200500450},
  doi          = {10.1002/star.200500450},
  volume       = {58},
  year         = {2006},
}