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Melatonin Prevents Early but Not Delayed Ventricular Fibrillation in the Experimental Porcine Model of Acute Ischemia

Tsvetkova, Alena S. ; Bernikova, Olesya G. ; Mikhaleva, Natalya J. ; Khramova, Darya S. ; Ovechkin, Alexey O. ; Demidova, Marina M. LU ; Platonov, Pyotr G. LU and Azarov, Jan E. (2020) In International Journal of Molecular Sciences 22(1).
Abstract

Antiarrhythmic effects of melatonin have been demonstrated ex vivo and in rodent models, but its action in a clinically relevant large mammalian model remains largely unknown. Objectives of the present study were to evaluate electrophysiological and antiarrhythmic effects of melatonin in a porcine model of acute myocardial infarction. Myocardial ischemia was induced by 40-min coronary occlusion in 25 anesthetized pigs. After ischemia onset, 12 animals received melatonin (4 mg/kg). 48 intramyocardial electrograms were recorded from left ventricular wall and interventricular septum (IVS). In each lead, activation time (AT) and repolarization time (RT) were determined. During ischemia, ATs and dispersion of repolarization (DOR = RTmax -... (More)

Antiarrhythmic effects of melatonin have been demonstrated ex vivo and in rodent models, but its action in a clinically relevant large mammalian model remains largely unknown. Objectives of the present study were to evaluate electrophysiological and antiarrhythmic effects of melatonin in a porcine model of acute myocardial infarction. Myocardial ischemia was induced by 40-min coronary occlusion in 25 anesthetized pigs. After ischemia onset, 12 animals received melatonin (4 mg/kg). 48 intramyocardial electrograms were recorded from left ventricular wall and interventricular septum (IVS). In each lead, activation time (AT) and repolarization time (RT) were determined. During ischemia, ATs and dispersion of repolarization (DOR = RTmax - RTmin) increased reaching maximal values by 3-5 and 20-25 min, respectively. Ventricular fibrillation (VF) incidence demonstrated no relations to redox state markers and was associated with increased DOR and delayed ATs (specifically, in an IVS base, an area adjacent to the ischemic zone) (p = 0.031). Melatonin prevented AT increase in the IVS base, (p < 0.001) precluding development of early VF (1-5 min, p = 0.016). VF occurrence in the delayed phase (17-40 min) where DOR was maximal was not modified by melatonin. Thus, melatonin-related enhancement of activation prevented development of early VF in the myocardial infarction model.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
depolarization, ischemia, melatonin, pig, repolarization, ventricular fibrillation
in
International Journal of Molecular Sciences
volume
22
issue
1
publisher
MDPI AG
external identifiers
  • pmid:33396934
  • scopus:85099332072
ISSN
1422-0067
DOI
10.3390/ijms22010328
language
English
LU publication?
yes
id
e325cac7-764a-49ab-adb0-50abce7ee11e
date added to LUP
2021-01-26 09:47:21
date last changed
2024-04-18 01:08:10
@article{e325cac7-764a-49ab-adb0-50abce7ee11e,
  abstract     = {{<p>Antiarrhythmic effects of melatonin have been demonstrated ex vivo and in rodent models, but its action in a clinically relevant large mammalian model remains largely unknown. Objectives of the present study were to evaluate electrophysiological and antiarrhythmic effects of melatonin in a porcine model of acute myocardial infarction. Myocardial ischemia was induced by 40-min coronary occlusion in 25 anesthetized pigs. After ischemia onset, 12 animals received melatonin (4 mg/kg). 48 intramyocardial electrograms were recorded from left ventricular wall and interventricular septum (IVS). In each lead, activation time (AT) and repolarization time (RT) were determined. During ischemia, ATs and dispersion of repolarization (DOR = RTmax - RTmin) increased reaching maximal values by 3-5 and 20-25 min, respectively. Ventricular fibrillation (VF) incidence demonstrated no relations to redox state markers and was associated with increased DOR and delayed ATs (specifically, in an IVS base, an area adjacent to the ischemic zone) (p = 0.031). Melatonin prevented AT increase in the IVS base, (p &lt; 0.001) precluding development of early VF (1-5 min, p = 0.016). VF occurrence in the delayed phase (17-40 min) where DOR was maximal was not modified by melatonin. Thus, melatonin-related enhancement of activation prevented development of early VF in the myocardial infarction model.</p>}},
  author       = {{Tsvetkova, Alena S. and Bernikova, Olesya G. and Mikhaleva, Natalya J. and Khramova, Darya S. and Ovechkin, Alexey O. and Demidova, Marina M. and Platonov, Pyotr G. and Azarov, Jan E.}},
  issn         = {{1422-0067}},
  keywords     = {{depolarization; ischemia; melatonin; pig; repolarization; ventricular fibrillation}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Melatonin Prevents Early but Not Delayed Ventricular Fibrillation in the Experimental Porcine Model of Acute Ischemia}},
  url          = {{http://dx.doi.org/10.3390/ijms22010328}},
  doi          = {{10.3390/ijms22010328}},
  volume       = {{22}},
  year         = {{2020}},
}