Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16S rRNA genes.

Olsson, Crister LU ; Ahrné, Siv LU ; Pettersson, B and Molin, Göran LU (2003) In International Journal of Food Microbiology 83(3). p.245-252
Abstract
The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows... (More)
The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows that the overall pattern of the initial and chill-stored pork flora, as shown by a molecular approach, was in agreement with results obtained in previous studies using traditional cultivation methods. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pork, Eperythrozoon, Diversity, 16S rDNA cloning
in
International Journal of Food Microbiology
volume
83
issue
3
pages
245 - 252
publisher
Elsevier
external identifiers
  • wos:000183055800002
  • scopus:0038078459
ISSN
0168-1605
DOI
10.1016/S0168-1605(02)00372-0
language
English
LU publication?
yes
id
e5be906e-dded-42a9-921c-90e0b4623249 (old id 128295)
date added to LUP
2016-04-01 11:43:06
date last changed
2025-10-14 09:07:15
@article{e5be906e-dded-42a9-921c-90e0b4623249,
  abstract     = {{The composition of the initial bacterial flora of pork and the development of the flora after storage at +4 °C for 4 days were analysed by amplification, cloning and sequencing of 16S rDNA. A total of 122 clones were obtained, with lengths of ≥400 nucleotides and ≥95% similarity to database sequences. Nineteen clones were similar to sequences in database not assigned to any genera. Fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones most resembling Acinetobacter and 17.3% resembling Staphylococcus and Macrococcus. After storage, the clones were composed of six different genera, with 44.3% resembling Pseudomonas, 17.1% resembling Aeromonas and only 14.3% resembling Acinetobacter. This study shows that the overall pattern of the initial and chill-stored pork flora, as shown by a molecular approach, was in agreement with results obtained in previous studies using traditional cultivation methods.}},
  author       = {{Olsson, Crister and Ahrné, Siv and Pettersson, B and Molin, Göran}},
  issn         = {{0168-1605}},
  keywords     = {{Pork; Eperythrozoon; Diversity; 16S rDNA cloning}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{245--252}},
  publisher    = {{Elsevier}},
  series       = {{International Journal of Food Microbiology}},
  title        = {{The bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16S rRNA genes.}},
  url          = {{http://dx.doi.org/10.1016/S0168-1605(02)00372-0}},
  doi          = {{10.1016/S0168-1605(02)00372-0}},
  volume       = {{83}},
  year         = {{2003}},
}