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Evidence that the antibiotic ciprofloxacin counteracts cyclosporine‐dependent suppression of cytokine production

Riesbeck, Kristian LU orcid ; Gullberg, Martin and Forsgren, Arne LU (1994) In Transplantation 57(2). p.267-272
Abstract

The fluoroquinolone antibiotic ciprofloxacin (cipro) has been reported to upregulate interleukin 2 and interferon-γ production in lectin-stimulated lymphocytes. The aim of this study was to elucidate whether cipro and the immunosuppressive agent CsA have antagonistic action on cytokine synthesis. Accumulation of IL-2 and IFN-γ protein and mRNA were analyzed in polyclonally (PHA or Con A) or alloantigen-stimulated human peripheral blood lymphocytes. CsA added simultaneously with PHA partially blocked cytokine synthesis. The present study also shows that cipro supplemented with CsA and PHA resulted in significant higher concentrations of IL-2 (up to 60 times) and IFN-γ (4.3 times) as compared with PHA and CsA alone. Similar results were... (More)

The fluoroquinolone antibiotic ciprofloxacin (cipro) has been reported to upregulate interleukin 2 and interferon-γ production in lectin-stimulated lymphocytes. The aim of this study was to elucidate whether cipro and the immunosuppressive agent CsA have antagonistic action on cytokine synthesis. Accumulation of IL-2 and IFN-γ protein and mRNA were analyzed in polyclonally (PHA or Con A) or alloantigen-stimulated human peripheral blood lymphocytes. CsA added simultaneously with PHA partially blocked cytokine synthesis. The present study also shows that cipro supplemented with CsA and PHA resulted in significant higher concentrations of IL-2 (up to 60 times) and IFN-γ (4.3 times) as compared with PHA and CsA alone. Similar results were obtained with primary mixed lymphocyte reactions. In parallel, a greater amount of IL-2 and IFN-γ mRNA was observed in lymphocytes incubated with both cipro and CsA as compared with CsA alone. Our results reveal that CsA-dependent inhibition of both IL-2 and IFN-γ expression is counteracted by high concentrations of cipro. These findings may be of importance in clinical transplantation.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transplantation
volume
57
issue
2
pages
267 - 272
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0028047850
  • pmid:8310519
ISSN
0041-1337
DOI
10.1097/00007890-199401001-00020
language
English
LU publication?
yes
id
e6a1ced6-64ee-4a76-8a76-d93828a376d7
date added to LUP
2019-03-29 11:46:28
date last changed
2024-05-15 03:38:57
@article{e6a1ced6-64ee-4a76-8a76-d93828a376d7,
  abstract     = {{<p>The fluoroquinolone antibiotic ciprofloxacin (cipro) has been reported to upregulate interleukin 2 and interferon-γ production in lectin-stimulated lymphocytes. The aim of this study was to elucidate whether cipro and the immunosuppressive agent CsA have antagonistic action on cytokine synthesis. Accumulation of IL-2 and IFN-γ protein and mRNA were analyzed in polyclonally (PHA or Con A) or alloantigen-stimulated human peripheral blood lymphocytes. CsA added simultaneously with PHA partially blocked cytokine synthesis. The present study also shows that cipro supplemented with CsA and PHA resulted in significant higher concentrations of IL-2 (up to 60 times) and IFN-γ (4.3 times) as compared with PHA and CsA alone. Similar results were obtained with primary mixed lymphocyte reactions. In parallel, a greater amount of IL-2 and IFN-γ mRNA was observed in lymphocytes incubated with both cipro and CsA as compared with CsA alone. Our results reveal that CsA-dependent inhibition of both IL-2 and IFN-γ expression is counteracted by high concentrations of cipro. These findings may be of importance in clinical transplantation.</p>}},
  author       = {{Riesbeck, Kristian and Gullberg, Martin and Forsgren, Arne}},
  issn         = {{0041-1337}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{267--272}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Transplantation}},
  title        = {{Evidence that the antibiotic ciprofloxacin counteracts cyclosporine‐dependent suppression of cytokine production}},
  url          = {{http://dx.doi.org/10.1097/00007890-199401001-00020}},
  doi          = {{10.1097/00007890-199401001-00020}},
  volume       = {{57}},
  year         = {{1994}},
}