Induced Growth and Maturation of the Gastrointestinal Tract After Phaseolus vulgaris Lectin Exposure in Suckling Rats
(2005) In Journal of Pediatric Gastroenterology and Nutrition - Jpgn 41(August). p.195-203- Abstract
- Objectives: In mammals, the postnatal development of the
gastrointestinal tract is characterized by vast structural and
functional changes. Using a suckling rat model, we investigated
whether red kidney bean lectin, phytohemagglutinin (PHA),
a potent gut mitogen in adult rats, can accelerate the growth and
maturation of the gastrointestinal tract.
Methods: At either 10 or 14 days of age, suckling rats were daily
gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.
At 1 or 3 days after this treatment, gastrointestinal organ growth,
intestinal morphology, disaccharidase pattern, macromolecular
absorption capacity, and pancreatic enzyme... (More) - Objectives: In mammals, the postnatal development of the
gastrointestinal tract is characterized by vast structural and
functional changes. Using a suckling rat model, we investigated
whether red kidney bean lectin, phytohemagglutinin (PHA),
a potent gut mitogen in adult rats, can accelerate the growth and
maturation of the gastrointestinal tract.
Methods: At either 10 or 14 days of age, suckling rats were daily
gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.
At 1 or 3 days after this treatment, gastrointestinal organ growth,
intestinal morphology, disaccharidase pattern, macromolecular
absorption capacity, and pancreatic enzyme contents were studied.
Results: After PHA exposure, increased small intestinal growth
and number of crypt cells were observed, whereas the proportion
of enterocytes with supranuclear vacuoles in the distal
intestine was decreased. The macromolecular absorption of the
markers bovine immunoglobulin (Ig)G and bovine serum
albumin and plasma levels of maternal IgG decreased, and
intestinal disaccharidases switched toward an adult-like pattern.
The pancreas weight and pancreatic protein and trypsin contents
increased. These changes were partly reversible when the PHA
treatment began at 10 days of age, but they persisted when the
treatment began at 14 days of age.
Conclusions: PHA induced enhanced growth and precocious
functional maturation of the gastrointestinal tract in suckling
rats. The effects persisted if the PHA treatment started at 14
days of age, but not before, suggesting an age dependent mechanism.
These findings may lead to a better understanding of
gastrointestinal maturation and constitute a basis for the treatment
of mammals having an immature gut. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1244631
- author
- Linderoth, Ann LU ; Biernat, Marzena ; Prykhodko, Olena LU ; Kornilovska, Iryna ; Pusztai, Arpad ; Pierzynowski, Stefan LU and Weström, Björn LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Endocytosis, Pancreas, Disaccharidases, Gut, Permeability
- in
- Journal of Pediatric Gastroenterology and Nutrition - Jpgn
- volume
- 41
- issue
- August
- pages
- 195 - 203
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000230885000008
- pmid:16056099
- scopus:23244456050
- ISSN
- 1536-4801
- language
- English
- LU publication?
- yes
- id
- e6eb3c62-ee54-40c9-a5d2-7dac9d3cda49 (old id 1244631)
- alternative location
- http://jpgn.org/pt/re/jpgn/pdfhandler.00005176-200508000-00008.pdf
- date added to LUP
- 2016-04-01 12:04:27
- date last changed
- 2022-01-26 22:24:05
@article{e6eb3c62-ee54-40c9-a5d2-7dac9d3cda49,
abstract = {{Objectives: In mammals, the postnatal development of the<br/><br>
gastrointestinal tract is characterized by vast structural and<br/><br>
functional changes. Using a suckling rat model, we investigated<br/><br>
whether red kidney bean lectin, phytohemagglutinin (PHA),<br/><br>
a potent gut mitogen in adult rats, can accelerate the growth and<br/><br>
maturation of the gastrointestinal tract.<br/><br>
Methods: At either 10 or 14 days of age, suckling rats were daily<br/><br>
gavage fed with PHA (0.05 mg/g body weight) or saline for 3 days.<br/><br>
At 1 or 3 days after this treatment, gastrointestinal organ growth,<br/><br>
intestinal morphology, disaccharidase pattern, macromolecular<br/><br>
absorption capacity, and pancreatic enzyme contents were studied.<br/><br>
Results: After PHA exposure, increased small intestinal growth<br/><br>
and number of crypt cells were observed, whereas the proportion<br/><br>
of enterocytes with supranuclear vacuoles in the distal<br/><br>
intestine was decreased. The macromolecular absorption of the<br/><br>
markers bovine immunoglobulin (Ig)G and bovine serum<br/><br>
albumin and plasma levels of maternal IgG decreased, and<br/><br>
intestinal disaccharidases switched toward an adult-like pattern.<br/><br>
The pancreas weight and pancreatic protein and trypsin contents<br/><br>
increased. These changes were partly reversible when the PHA<br/><br>
treatment began at 10 days of age, but they persisted when the<br/><br>
treatment began at 14 days of age.<br/><br>
Conclusions: PHA induced enhanced growth and precocious<br/><br>
functional maturation of the gastrointestinal tract in suckling<br/><br>
rats. The effects persisted if the PHA treatment started at 14<br/><br>
days of age, but not before, suggesting an age dependent mechanism.<br/><br>
These findings may lead to a better understanding of<br/><br>
gastrointestinal maturation and constitute a basis for the treatment<br/><br>
of mammals having an immature gut.}},
author = {{Linderoth, Ann and Biernat, Marzena and Prykhodko, Olena and Kornilovska, Iryna and Pusztai, Arpad and Pierzynowski, Stefan and Weström, Björn}},
issn = {{1536-4801}},
keywords = {{Endocytosis; Pancreas; Disaccharidases; Gut; Permeability}},
language = {{eng}},
number = {{August}},
pages = {{195--203}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Journal of Pediatric Gastroenterology and Nutrition - Jpgn}},
title = {{Induced Growth and Maturation of the Gastrointestinal Tract After Phaseolus vulgaris Lectin Exposure in Suckling Rats}},
url = {{http://jpgn.org/pt/re/jpgn/pdfhandler.00005176-200508000-00008.pdf}},
volume = {{41}},
year = {{2005}},
}