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Detection of circulating tumor cells and circulating tumor DNA before and after mammographic breast compression in a cohort of breast cancer patients scheduled for neoadjuvant treatment

Förnvik, Daniel LU ; Aaltonen, Kristina LU ; Chen, Yilun LU ; George, Anthony LU ; Brueffer, Christian LU ; Rigo, Robert LU ; Loman, Niklas LU ; Saal, Lao LU and Rydén, Lisa LU (2019) In Breast Cancer Research and Treatment 177(2). p.447-455
Abstract
Purpose
It is not known if mammographic breast compression of a primary tumor causes shedding of tumor cells into the circulatory system. Little is known about how the detection of circulating biomarkers such as circulating tumor cells (CTCs) or circulating tumor DNA (ctDNA) is affected by breast compression intervention.

Methods
CTCs and ctDNA were analyzed in blood samples collected before and after breast compression in 31 patients with primary breast cancer scheduled for neoadjuvant therapy. All patients had a central venous access to allow administration of intravenous neoadjuvant chemotherapy, which enabled blood collection from superior vena cava, draining the breasts, in addition to sampling from a peripheral... (More)
Purpose
It is not known if mammographic breast compression of a primary tumor causes shedding of tumor cells into the circulatory system. Little is known about how the detection of circulating biomarkers such as circulating tumor cells (CTCs) or circulating tumor DNA (ctDNA) is affected by breast compression intervention.

Methods
CTCs and ctDNA were analyzed in blood samples collected before and after breast compression in 31 patients with primary breast cancer scheduled for neoadjuvant therapy. All patients had a central venous access to allow administration of intravenous neoadjuvant chemotherapy, which enabled blood collection from superior vena cava, draining the breasts, in addition to sampling from a peripheral vein.

Results
CTC and ctDNA positivity was seen in 26% and 65% of the patients, respectively. There was a significant increase of ctDNA after breast compression in central blood (p = 0.01), not observed in peripheral testing. No increase related with breast compression was observed for CTC. ctDNA positivity was associated with older age (p = 0.05), and ctDNA increase after breast compression was associated with high Ki67 proliferating tumors (p = 0.04). CTCs were more abundant in central compared to peripheral blood samples (p = 0.04).

Conclusions
There was no significant release of CTCs after mammographic breast compression but more CTCs were present in central compared to peripheral blood. No significant difference between central and peripheral levels of ctDNA was observed. The small average increase in ctDNA after breast compression is unlikely to be clinically relevant. The results give support for mammography as a safe procedure from the point of view of CTC and ctDNA shedding to the blood circulation. The results may have implications for the standardization of sampling procedures for circulating tumor markers. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer, breast cancer, liquid biopsy, Circulating tumor cells (CTCs), circulating tumor DNA
in
Breast Cancer Research and Treatment
volume
177
issue
2
pages
447 - 455
publisher
Springer
external identifiers
  • scopus:85068046912
ISSN
1573-7217
DOI
10.1007/s10549-019-05326-5
language
English
LU publication?
yes
id
e978e6ee-0cf6-4c59-b0c4-95a22f11908b
date added to LUP
2019-06-26 11:28:14
date last changed
2019-08-23 15:49:33
@article{e978e6ee-0cf6-4c59-b0c4-95a22f11908b,
  abstract     = {Purpose<br/>It is not known if mammographic breast compression of a primary tumor causes shedding of tumor cells into the circulatory system. Little is known about how the detection of circulating biomarkers such as circulating tumor cells (CTCs) or circulating tumor DNA (ctDNA) is affected by breast compression intervention.<br/><br/>Methods<br/>CTCs and ctDNA were analyzed in blood samples collected before and after breast compression in 31 patients with primary breast cancer scheduled for neoadjuvant therapy. All patients had a central venous access to allow administration of intravenous neoadjuvant chemotherapy, which enabled blood collection from superior vena cava, draining the breasts, in addition to sampling from a peripheral vein.<br/><br/>Results<br/>CTC and ctDNA positivity was seen in 26% and 65% of the patients, respectively. There was a significant increase of ctDNA after breast compression in central blood (p = 0.01), not observed in peripheral testing. No increase related with breast compression was observed for CTC. ctDNA positivity was associated with older age (p = 0.05), and ctDNA increase after breast compression was associated with high Ki67 proliferating tumors (p = 0.04). CTCs were more abundant in central compared to peripheral blood samples (p = 0.04).<br/><br/>Conclusions<br/>There was no significant release of CTCs after mammographic breast compression but more CTCs were present in central compared to peripheral blood. No significant difference between central and peripheral levels of ctDNA was observed. The small average increase in ctDNA after breast compression is unlikely to be clinically relevant. The results give support for mammography as a safe procedure from the point of view of CTC and ctDNA shedding to the blood circulation. The results may have implications for the standardization of sampling procedures for circulating tumor markers.},
  author       = {Förnvik, Daniel and Aaltonen, Kristina and Chen, Yilun and George, Anthony and Brueffer, Christian and Rigo, Robert and Loman, Niklas and Saal, Lao and Rydén, Lisa},
  issn         = {1573-7217},
  keyword      = {cancer,breast cancer,liquid biopsy,Circulating tumor cells (CTCs),circulating tumor DNA},
  language     = {eng},
  month        = {06},
  number       = {2},
  pages        = {447--455},
  publisher    = {Springer},
  series       = {Breast Cancer Research and Treatment},
  title        = {Detection of circulating tumor cells and circulating tumor DNA before and after mammographic breast compression in a cohort of breast cancer patients scheduled for neoadjuvant treatment},
  url          = {http://dx.doi.org/10.1007/s10549-019-05326-5},
  volume       = {177},
  year         = {2019},
}