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Synthesis and application of PEGylated tracer particles for measuring protein solution viscosities using Dynamic Light Scattering-based microrheology

Garting, Tommy LU and Stradner, Anna LU (2019) In Colloids and Surfaces B: Biointerfaces 181. p.516-523
Abstract

The measurement of flow properties, such as the zero shear viscosity, of protein solutions is of paramount importance for many applications such as pharmaceutical formulations, where the syringeability of physiologically effective doses is a key property. However, the determination of these properties with classical rheological methods is often challenging due to e.g. detrimental surface effects or simply the lack of sufficient material. A possible alternative is Dynamic Light Scattering-based microrheology, where the Brownian motion of tracer particles embedded in the protein solution is monitored to access the zero shear viscosity of the sample. The prime advantages of this method compared to classical rheology are the absence of... (More)

The measurement of flow properties, such as the zero shear viscosity, of protein solutions is of paramount importance for many applications such as pharmaceutical formulations, where the syringeability of physiologically effective doses is a key property. However, the determination of these properties with classical rheological methods is often challenging due to e.g. detrimental surface effects or simply the lack of sufficient material. A possible alternative is Dynamic Light Scattering-based microrheology, where the Brownian motion of tracer particles embedded in the protein solution is monitored to access the zero shear viscosity of the sample. The prime advantages of this method compared to classical rheology are the absence of disturbing surface effects and the up to two orders of magnitude smaller protein quantities needed for an entire concentration series. This Protocol provides a detailed description of the synthesis of sterically stabilized tracer particles with surface and overall particle properties specifically designed to investigate the viscosity of protein solutions up to concentrations close to the arrest transition. These particles are tailored to avoid protein-particle as well as particle-particle aggregation at various sample conditions and thus allow for an artifact-free application of Dynamic Light Scattering-based tracer microrheology to determine the flow behaviour of biological samples. The Protocol concludes with step by step instructions for the characterization of protein solutions using a combination of the tracer particles and an advanced dynamic light scattering technique yielding the concentration-dependent zero shear viscosity.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dynamic Light Scattering, High Molecular Weight PEG, Inert Tracer Particles, Microrheology, Protein Solutions, Steric Stabilization, Zero Shear Viscosity
in
Colloids and Surfaces B: Biointerfaces
volume
181
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85066818645
  • pmid:31181434
ISSN
0927-7765
DOI
10.1016/j.colsurfb.2019.05.059
language
English
LU publication?
yes
id
e98acda0-3850-41b2-b917-b06e40a196bf
date added to LUP
2019-06-24 15:08:50
date last changed
2020-01-13 02:05:58
@article{e98acda0-3850-41b2-b917-b06e40a196bf,
  abstract     = {<p>The measurement of flow properties, such as the zero shear viscosity, of protein solutions is of paramount importance for many applications such as pharmaceutical formulations, where the syringeability of physiologically effective doses is a key property. However, the determination of these properties with classical rheological methods is often challenging due to e.g. detrimental surface effects or simply the lack of sufficient material. A possible alternative is Dynamic Light Scattering-based microrheology, where the Brownian motion of tracer particles embedded in the protein solution is monitored to access the zero shear viscosity of the sample. The prime advantages of this method compared to classical rheology are the absence of disturbing surface effects and the up to two orders of magnitude smaller protein quantities needed for an entire concentration series. This Protocol provides a detailed description of the synthesis of sterically stabilized tracer particles with surface and overall particle properties specifically designed to investigate the viscosity of protein solutions up to concentrations close to the arrest transition. These particles are tailored to avoid protein-particle as well as particle-particle aggregation at various sample conditions and thus allow for an artifact-free application of Dynamic Light Scattering-based tracer microrheology to determine the flow behaviour of biological samples. The Protocol concludes with step by step instructions for the characterization of protein solutions using a combination of the tracer particles and an advanced dynamic light scattering technique yielding the concentration-dependent zero shear viscosity.</p>},
  author       = {Garting, Tommy and Stradner, Anna},
  issn         = {0927-7765},
  language     = {eng},
  pages        = {516--523},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Synthesis and application of PEGylated tracer particles for measuring protein solution viscosities using Dynamic Light Scattering-based microrheology},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2019.05.059},
  doi          = {10.1016/j.colsurfb.2019.05.059},
  volume       = {181},
  year         = {2019},
}