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Leaf mineral content govern microbial community structure in the phyllosphere of spinach (Spinacia oleracea) and rocket (Diplotaxis tenuifolia)

Darlison, Julia; Mogren, Lars; Rosberg, Anna Karin; Grudén, Maria; Minet, Antoine; Liné, Clarisse; Mieli, Morgane; Bengtsson, Torbjörn; Håkansson, Åsa LU and Uhlig, Elisabeth LU , et al. (2019) In The Science of the total environment 675. p.501-512
Abstract

The plant microbiome is an important factor for plant health and productivity. While the impact of nitrogen (N) availability for plant growth and development is well established, its influence on the microbial phyllosphere community structure is unknown. We hypothesize that nitrogen impacts the growth and abundance of several microorganisms on the leaf surface. The bacterial and fungal communities of baby leaf spinach (Spinacia oleracea), and rocket (Diplotaxis tenuifolia) were investigated in a field trial for two years in a commercial setting. Nitrogen fertilizer was tested in four doses (basic nitrogen, basic + suboptimal, basic + commercial, basic + excess) with six replicates in each. Culture-independent (Illumina sequencing) and... (More)

The plant microbiome is an important factor for plant health and productivity. While the impact of nitrogen (N) availability for plant growth and development is well established, its influence on the microbial phyllosphere community structure is unknown. We hypothesize that nitrogen impacts the growth and abundance of several microorganisms on the leaf surface. The bacterial and fungal communities of baby leaf spinach (Spinacia oleracea), and rocket (Diplotaxis tenuifolia) were investigated in a field trial for two years in a commercial setting. Nitrogen fertilizer was tested in four doses (basic nitrogen, basic + suboptimal, basic + commercial, basic + excess) with six replicates in each. Culture-independent (Illumina sequencing) and culture-dependent (viable count and identification of bacterial isolates) community studies were combined with monitoring of plant physiology and site weather conditions. This study found that alpha diversity of bacterial communities decreased in response to increasing nitrogen fertilizer dose, whereas viable counts showed no differences. Correspondingly, fungal communities of the spinach phyllosphere showed a decreasing pattern, whereas the decreasing diversity of fungal communities of rocket was not significant. Plant species and effects of annual variations on microbiome structure were observed for bacterial and fungal communities on both spinach and rocket. This study provides novel insights on the impact of nitrogen fertilizer regime on a nutrient scarce habitat, the phyllosphere.

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publication status
published
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keywords
Leafy vegetables, Metagenomics, Microbial diversity, Nitrogen fertilizer, Phyllosphere, Plant-microbe interactions
in
The Science of the total environment
volume
675
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:85066057218
ISSN
1879-1026
DOI
10.1016/j.scitotenv.2019.04.254
language
English
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yes
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ea830701-48c5-4545-baed-1103fa63a4fe
date added to LUP
2019-06-11 10:02:55
date last changed
2019-06-25 03:55:18
@article{ea830701-48c5-4545-baed-1103fa63a4fe,
  abstract     = {<p>The plant microbiome is an important factor for plant health and productivity. While the impact of nitrogen (N) availability for plant growth and development is well established, its influence on the microbial phyllosphere community structure is unknown. We hypothesize that nitrogen impacts the growth and abundance of several microorganisms on the leaf surface. The bacterial and fungal communities of baby leaf spinach (Spinacia oleracea), and rocket (Diplotaxis tenuifolia) were investigated in a field trial for two years in a commercial setting. Nitrogen fertilizer was tested in four doses (basic nitrogen, basic + suboptimal, basic + commercial, basic + excess) with six replicates in each. Culture-independent (Illumina sequencing) and culture-dependent (viable count and identification of bacterial isolates) community studies were combined with monitoring of plant physiology and site weather conditions. This study found that alpha diversity of bacterial communities decreased in response to increasing nitrogen fertilizer dose, whereas viable counts showed no differences. Correspondingly, fungal communities of the spinach phyllosphere showed a decreasing pattern, whereas the decreasing diversity of fungal communities of rocket was not significant. Plant species and effects of annual variations on microbiome structure were observed for bacterial and fungal communities on both spinach and rocket. This study provides novel insights on the impact of nitrogen fertilizer regime on a nutrient scarce habitat, the phyllosphere.</p>},
  author       = {Darlison, Julia and Mogren, Lars and Rosberg, Anna Karin and Grudén, Maria and Minet, Antoine and Liné, Clarisse and Mieli, Morgane and Bengtsson, Torbjörn and Håkansson, Åsa and Uhlig, Elisabeth and Becher, Paul G. and Karlsson, Maria and Alsanius, Beatrix W.},
  issn         = {1879-1026},
  keyword      = {Leafy vegetables,Metagenomics,Microbial diversity,Nitrogen fertilizer,Phyllosphere,Plant-microbe interactions},
  language     = {eng},
  pages        = {501--512},
  publisher    = {Elsevier},
  series       = {The Science of the total environment},
  title        = {Leaf mineral content govern microbial community structure in the phyllosphere of spinach (Spinacia oleracea) and rocket (Diplotaxis tenuifolia)},
  url          = {http://dx.doi.org/10.1016/j.scitotenv.2019.04.254},
  volume       = {675},
  year         = {2019},
}