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Aqueous two-phase affinity partitioning of biotinylated liposomes using neutral avidin as affinity ligand

Ekblad, Lars LU orcid ; Kernbichler, Judith and Jergil, Bengt LU (1998) In Journal of Chromatography A 815(2). p.189-195
Abstract
Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome,... (More)
Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome, coupled via a C6 spacer arm, was required to redistribute the liposomes to the bottom phase. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phospholipids
in
Journal of Chromatography A
volume
815
issue
2
pages
189 - 195
publisher
Elsevier
external identifiers
  • scopus:0031857414
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)00433-6
language
English
LU publication?
yes
id
ead80a69-8f5e-443b-9eca-d064b417835f (old id 125506)
date added to LUP
2016-04-01 16:45:09
date last changed
2025-10-14 12:27:31
@article{ead80a69-8f5e-443b-9eca-d064b417835f,
  abstract     = {{Biotinylated small unilamellar liposomes were affinity partitioned in an aqueous poly(ethylene glycol)-dextran two-phase system using avidin coupled to dextran as affinity ligand. In the absence of affinity ligand more than 90% of the liposomes partitioned in the poly(ethylene glycol)-rich top phase, whereas in its presence more than 95% partitioned in the dextran-rich bottom phase. For this redistribution to occur 10 mM and above of lithium sulphate, or other appropriate salts, had to be added to the two-phase system. Without added salt the liposomes with complexed avidin-dextran instead partitioned in the top phase. An extended mixing time for the system was required for maximum redistribution. Less than two biotin residues per liposome, coupled via a C6 spacer arm, was required to redistribute the liposomes to the bottom phase.}},
  author       = {{Ekblad, Lars and Kernbichler, Judith and Jergil, Bengt}},
  issn         = {{0021-9673}},
  keywords     = {{Phospholipids}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{189--195}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Aqueous two-phase affinity partitioning of biotinylated liposomes using neutral avidin as affinity ligand}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(98)00433-6}},
  doi          = {{10.1016/S0021-9673(98)00433-6}},
  volume       = {{815}},
  year         = {{1998}},
}