Kir3 channel blockade in the cerebellar cortex suppresses performance of classically conditioned Purkinje cell responses
(2020) In Scientific Reports 10(1).- Abstract
In the eyeblink conditioning paradigm, cerebellar Purkinje cells learn to respond to the conditional stimulus with an adaptively timed pause in its spontaneous firing. Evidence suggests that the pause is elicited by glutamate released from parallel fibers and acting on metabotropic receptors (mGluR7) which initiates a delayed-onset suppression of firing. We suggested that G protein activation of hyperpolarizing Kir3 channels (or ‘GIRK’, G protein-coupled inwardly-rectifying K+ channels) could be part of such a mechanism. Application of the Kir3 antagonist Tertiapin-LQ locally in the superficial layers of the cerebellar cortex in decerebrate ferrets suppressed normal performance of Purkinje cell pause... (More)
In the eyeblink conditioning paradigm, cerebellar Purkinje cells learn to respond to the conditional stimulus with an adaptively timed pause in its spontaneous firing. Evidence suggests that the pause is elicited by glutamate released from parallel fibers and acting on metabotropic receptors (mGluR7) which initiates a delayed-onset suppression of firing. We suggested that G protein activation of hyperpolarizing Kir3 channels (or ‘GIRK’, G protein-coupled inwardly-rectifying K+ channels) could be part of such a mechanism. Application of the Kir3 antagonist Tertiapin-LQ locally in the superficial layers of the cerebellar cortex in decerebrate ferrets suppressed normal performance of Purkinje cell pause responses to the conditional stimulus. Importantly, there was no detectable effect on spontaneous firing. These findings suggest that intact functioning of Kir3 channels in the cerebellar cortex is required for normal conditioned Purkinje cell responses.
(Less)
- author
- Johansson, Fredrik LU and Hesslow, Germund LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- article number
- 15654
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85091445435
- pmid:32973240
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-020-72581-8
- language
- English
- LU publication?
- yes
- id
- eb047c61-ef19-44f4-8a16-46347d8e7387
- date added to LUP
- 2020-10-22 12:54:43
- date last changed
- 2024-04-03 15:52:24
@article{eb047c61-ef19-44f4-8a16-46347d8e7387,
abstract = {{<p>In the eyeblink conditioning paradigm, cerebellar Purkinje cells learn to respond to the conditional stimulus with an adaptively timed pause in its spontaneous firing. Evidence suggests that the pause is elicited by glutamate released from parallel fibers and acting on metabotropic receptors (mGluR7) which initiates a delayed-onset suppression of firing. We suggested that G protein activation of hyperpolarizing K<sub>ir</sub>3 channels (or ‘GIRK’, G protein-coupled inwardly-rectifying K<sup>+</sup> channels) could be part of such a mechanism. Application of the K<sub>ir</sub>3 antagonist Tertiapin-LQ locally in the superficial layers of the cerebellar cortex in decerebrate ferrets suppressed normal performance of Purkinje cell pause responses to the conditional stimulus. Importantly, there was no detectable effect on spontaneous firing. These findings suggest that intact functioning of K<sub>ir</sub>3 channels in the cerebellar cortex is required for normal conditioned Purkinje cell responses.</p>}},
author = {{Johansson, Fredrik and Hesslow, Germund}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Kir3 channel blockade in the cerebellar cortex suppresses performance of classically conditioned Purkinje cell responses}},
url = {{http://dx.doi.org/10.1038/s41598-020-72581-8}},
doi = {{10.1038/s41598-020-72581-8}},
volume = {{10}},
year = {{2020}},
}