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Fibroblasts Cultured on Nanowires Exhibit Low Motility, Impaired Cell Division, and DNA Damage.

Persson, Henrik LU ; Købler, Carsten; Mølhave, Kristian; Samuelson, Lars LU ; Tegenfeldt, Jonas LU ; Oredsson, Stina LU and Prinz, Christelle LU (2013) In Small 9(23). p.4006-4016
Abstract
Nanowires are commonly used as tools for interfacing living cells, acting as biomolecule-delivery vectors or electrodes. It is generally assumed that the small size of the nanowires ensures a minimal cellular perturbation, yet the effects of nanowires on cell migration and proliferation remain largely unknown. Fibroblast behaviour on vertical nanowire arrays is investigated, and it is shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration. Using focused ion beam milling and scanning electron microscopy, highly curved but intact nuclear membranes are observed, showing no direct contact between the... (More)
Nanowires are commonly used as tools for interfacing living cells, acting as biomolecule-delivery vectors or electrodes. It is generally assumed that the small size of the nanowires ensures a minimal cellular perturbation, yet the effects of nanowires on cell migration and proliferation remain largely unknown. Fibroblast behaviour on vertical nanowire arrays is investigated, and it is shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration. Using focused ion beam milling and scanning electron microscopy, highly curved but intact nuclear membranes are observed, showing no direct contact between the nanowires and the DNA. The nanowires possibly induce cellular stress and high respiration rates, which trigger the formation of ROS, which in turn results in DNA damage. These results are important guidelines to the design and interpretation of experiments involving nanowire-based transfection and electrical characterization of living cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Small
volume
9
issue
23
pages
4006 - 4016
publisher
John Wiley & Sons
external identifiers
  • wos:000331282400016
  • pmid:23813871
  • scopus:84879545715
ISSN
1613-6829
DOI
10.1002/smll.201300644
language
English
LU publication?
yes
id
ec1abf61-18dc-4405-8a90-2a502073887e (old id 3956322)
date added to LUP
2013-08-05 13:24:50
date last changed
2019-10-20 03:12:30
@article{ec1abf61-18dc-4405-8a90-2a502073887e,
  abstract     = {Nanowires are commonly used as tools for interfacing living cells, acting as biomolecule-delivery vectors or electrodes. It is generally assumed that the small size of the nanowires ensures a minimal cellular perturbation, yet the effects of nanowires on cell migration and proliferation remain largely unknown. Fibroblast behaviour on vertical nanowire arrays is investigated, and it is shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration. Using focused ion beam milling and scanning electron microscopy, highly curved but intact nuclear membranes are observed, showing no direct contact between the nanowires and the DNA. The nanowires possibly induce cellular stress and high respiration rates, which trigger the formation of ROS, which in turn results in DNA damage. These results are important guidelines to the design and interpretation of experiments involving nanowire-based transfection and electrical characterization of living cells.},
  author       = {Persson, Henrik and Købler, Carsten and Mølhave, Kristian and Samuelson, Lars and Tegenfeldt, Jonas and Oredsson, Stina and Prinz, Christelle},
  issn         = {1613-6829},
  language     = {eng},
  number       = {23},
  pages        = {4006--4016},
  publisher    = {John Wiley & Sons},
  series       = {Small},
  title        = {Fibroblasts Cultured on Nanowires Exhibit Low Motility, Impaired Cell Division, and DNA Damage.},
  url          = {http://dx.doi.org/10.1002/smll.201300644},
  volume       = {9},
  year         = {2013},
}