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Comparison of Three Different TTF-1 Clones in Resected Primary Lung Cancer and Epithelial Pulmonary Metastases

Vidarsdottir, Halla LU ; Tran, Lena; Nodin, Björn LU ; Jirström, Karin LU ; Planck, Maria LU ; Mattsson, Johanna S.M.; Botling, Johan; Micke, Patrick; Jönsson, Per LU and Brunnström, Hans LU (2018) In American Journal of Clinical Pathology 150(6). p.533-544
Abstract

Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.

Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.

Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and... (More)

Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.

Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.

Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas.

Conclusions: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Clinical Pathology
volume
150
issue
6
pages
12 pages
publisher
American Society for Clinical Pathology
external identifiers
  • scopus:85055604246
ISSN
1943-7722
DOI
10.1093/ajcp/aqy083
language
English
LU publication?
yes
id
ee7749c8-23a1-4a20-92ec-5fe1a0c89c14
date added to LUP
2018-11-15 12:01:53
date last changed
2019-02-20 11:36:07
@article{ee7749c8-23a1-4a20-92ec-5fe1a0c89c14,
  abstract     = {<p>Objectives: Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.</p><p>Methods: TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.</p><p>Results: Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas.</p><p>Conclusions: TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.</p>},
  author       = {Vidarsdottir, Halla and Tran, Lena and Nodin, Björn and Jirström, Karin and Planck, Maria and Mattsson, Johanna S.M. and Botling, Johan and Micke, Patrick and Jönsson, Per and Brunnström, Hans},
  issn         = {1943-7722},
  language     = {eng},
  number       = {6},
  pages        = {533--544},
  publisher    = {American Society for Clinical Pathology},
  series       = {American Journal of Clinical Pathology},
  title        = {Comparison of Three Different TTF-1 Clones in Resected Primary Lung Cancer and Epithelial Pulmonary Metastases},
  url          = {http://dx.doi.org/10.1093/ajcp/aqy083},
  volume       = {150},
  year         = {2018},
}