Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood
Pierzynowski, Stefan G. LU ; Wychowański, Piotr ; Szczesny, Wiesław ; Galloto, Robert ; Zaworski, Kamil LU ; Szkopek, Dominika ; Woliński, Jarosław LU ; Donaldson, Janine and Pierzynowska, Kateryna LU
(2025)
In Scientific Reports
15(1).
- Abstract
The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic... (More)
The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic importance of protein digestion end products in health and disease.
(Less)
- author
- Pierzynowski, Stefan G.
LU
; Wychowański, Piotr
; Szczesny, Wiesław
; Galloto, Robert
; Zaworski, Kamil
LU
; Szkopek, Dominika
; Woliński, Jarosław
LU
; Donaldson, Janine
and Pierzynowska, Kateryna
LU
- organization
- publishing date
- 2025-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Algorithm, Dipeptides, Mixed meal tolerance test, Protein digestion end products, Revisited ninhydrin method, Tripeptides
- in
- Scientific Reports
- volume
- 15
- issue
- 1
- article number
- 19704
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:40467756
- scopus:105007521041
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-025-04625-w
- language
- English
- LU publication?
- yes
- id
- efa5c633-f972-4082-8c30-6202eeb8736d
- date added to LUP
- 2025-10-28 16:16:23
- date last changed
- 2025-10-29 03:00:05
@article{efa5c633-f972-4082-8c30-6202eeb8736d,
abstract = {{<p>The recognition of amine groups by ninhydrin, along with a simple mathematical algorithm, showed that di- and tripeptides derived from dietary protein are the major end products of protein digestion entering the blood postprandially. There are thousands of oligopeptides appearing in the gut during protein digestion. However, the presented study on a pig model clearly shows that peptides longer than tri-amino acid peptides do not appear postprandially in the blood in nutritional amounts. We hypothesize that the measurement of postprandial free amino acids, di- and tripeptides and proportions between these components could be a useful, desirable tool both in laboratory and clinical practice, which could be used to determine the metabolic importance of protein digestion end products in health and disease.</p>}},
author = {{Pierzynowski, Stefan G. and Wychowański, Piotr and Szczesny, Wiesław and Galloto, Robert and Zaworski, Kamil and Szkopek, Dominika and Woliński, Jarosław and Donaldson, Janine and Pierzynowska, Kateryna}},
issn = {{2045-2322}},
keywords = {{Algorithm; Dipeptides; Mixed meal tolerance test; Protein digestion end products; Revisited ninhydrin method; Tripeptides}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Algorithm and ninhydrin method allow for measurement of the postprandial appearance of peptides in blood}},
url = {{http://dx.doi.org/10.1038/s41598-025-04625-w}},
doi = {{10.1038/s41598-025-04625-w}},
volume = {{15}},
year = {{2025}},
}