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Changes in paraurethral connective tissue at menopause are counteracted by estrogen

Falconer, C; Ekman-Ordeberg, G; Ulmsten, U; Westergren-Thorsson, G LU ; Barchan, K and Malmström, A LU (1996) In Maturitas 24(3). p.197-204
Abstract

OBJECTIVE: To study whether the transition to menopause is accompanied by changes in the paraurethral connective tissue and if these changes are modified by estrogen replacement therapy.

STUDY DESIGN: Biopsies were obtained from the paraurethral tissue from 34 women; 12 menstruating, 14 postmenopausal without estrogen treatment, and 8 with estrogen treatment. Collagen concentration and collagen extractability by pepsin digestion were measured. Proteoglycan composition and concentration were analysed using Alcian blue. The mRNA levels for collagen I and III, the small proteoglycans (PGS) decorin and biglycan, and the large proteoglycan versican, were estimated.

RESULTS: The paraurethral biopsies consisted of fibrous... (More)

OBJECTIVE: To study whether the transition to menopause is accompanied by changes in the paraurethral connective tissue and if these changes are modified by estrogen replacement therapy.

STUDY DESIGN: Biopsies were obtained from the paraurethral tissue from 34 women; 12 menstruating, 14 postmenopausal without estrogen treatment, and 8 with estrogen treatment. Collagen concentration and collagen extractability by pepsin digestion were measured. Proteoglycan composition and concentration were analysed using Alcian blue. The mRNA levels for collagen I and III, the small proteoglycans (PGS) decorin and biglycan, and the large proteoglycan versican, were estimated.

RESULTS: The paraurethral biopsies consisted of fibrous connective tissue, with collagen fibers as dominating structure. Several proteoglycans were identified; versican, heparansulphate proteoglycans, biglycan and decorin. The small proteoglycan decorin represented 85% of all proteoglycans. The collagen concentration was almost doubled in postmenopausal biopsies compared to premenopausal. The collagen fibril organization was also changed with higher cross-linking after menopause whereas the amount and the composition of the proteoglycans were unchanged. The proteoglycan/collagen ratio was significantly decreased. Estrogen replacement therapy resulted in decreased collagen concentration, decreased cross-linking of the collagen and reversal of the PGS/collagen ratio to almost premenopausal level. The therapy resulted in increased levels of mRNA for collagen I and III which suggests that the changes are due to an increased turnover.

CONCLUSION: The decrease in estrogen levels at menopause results in a connective tissue with different qualities after menopause. Estrogen replacement therapy tends to restore the metabolism of the genitourinary connective tissue to premenopausal conditions.

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Adult, Aged, Alcian Blue, Biglycan, Biopsy, Chondroitin Sulfate Proteoglycans, Collagen, Coloring Agents, Connective Tissue, Decorin, Dermatan Sulfate, Estrogen Replacement Therapy, Estrogens, Extracellular Matrix Proteins, Female, Heparitin Sulfate, Humans, Lectins, Lectins, C-Type, Menopause, Menstruation, Middle Aged, Pepsin A, Postmenopause, Premenopause, Proteoglycans, RNA, Messenger, Urethra, Versicans, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Maturitas
volume
24
issue
3
pages
197 - 204
publisher
Elsevier
external identifiers
  • scopus:0030199737
ISSN
0378-5122
DOI
10.1016/S0378-5122(96)82010-X
language
English
LU publication?
yes
id
efb4e7dd-4778-4065-b2f3-beb62ae8ce98
date added to LUP
2017-06-27 14:19:37
date last changed
2017-07-02 05:03:55
@article{efb4e7dd-4778-4065-b2f3-beb62ae8ce98,
  abstract     = {<p>OBJECTIVE: To study whether the transition to menopause is accompanied by changes in the paraurethral connective tissue and if these changes are modified by estrogen replacement therapy.</p><p>STUDY DESIGN: Biopsies were obtained from the paraurethral tissue from 34 women; 12 menstruating, 14 postmenopausal without estrogen treatment, and 8 with estrogen treatment. Collagen concentration and collagen extractability by pepsin digestion were measured. Proteoglycan composition and concentration were analysed using Alcian blue. The mRNA levels for collagen I and III, the small proteoglycans (PGS) decorin and biglycan, and the large proteoglycan versican, were estimated.</p><p>RESULTS: The paraurethral biopsies consisted of fibrous connective tissue, with collagen fibers as dominating structure. Several proteoglycans were identified; versican, heparansulphate proteoglycans, biglycan and decorin. The small proteoglycan decorin represented 85% of all proteoglycans. The collagen concentration was almost doubled in postmenopausal biopsies compared to premenopausal. The collagen fibril organization was also changed with higher cross-linking after menopause whereas the amount and the composition of the proteoglycans were unchanged. The proteoglycan/collagen ratio was significantly decreased. Estrogen replacement therapy resulted in decreased collagen concentration, decreased cross-linking of the collagen and reversal of the PGS/collagen ratio to almost premenopausal level. The therapy resulted in increased levels of mRNA for collagen I and III which suggests that the changes are due to an increased turnover.</p><p>CONCLUSION: The decrease in estrogen levels at menopause results in a connective tissue with different qualities after menopause. Estrogen replacement therapy tends to restore the metabolism of the genitourinary connective tissue to premenopausal conditions.</p>},
  author       = {Falconer, C and Ekman-Ordeberg, G and Ulmsten, U and Westergren-Thorsson, G and Barchan, K and Malmström, A},
  issn         = {0378-5122},
  keyword      = {Adult,Aged,Alcian Blue,Biglycan,Biopsy,Chondroitin Sulfate Proteoglycans,Collagen,Coloring Agents,Connective Tissue,Decorin,Dermatan Sulfate,Estrogen Replacement Therapy,Estrogens,Extracellular Matrix Proteins,Female,Heparitin Sulfate,Humans,Lectins,Lectins, C-Type,Menopause,Menstruation,Middle Aged,Pepsin A,Postmenopause,Premenopause,Proteoglycans,RNA, Messenger,Urethra,Versicans,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {197--204},
  publisher    = {Elsevier},
  series       = {Maturitas},
  title        = {Changes in paraurethral connective tissue at menopause are counteracted by estrogen},
  url          = {http://dx.doi.org/10.1016/S0378-5122(96)82010-X},
  volume       = {24},
  year         = {1996},
}