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Comprehensive Proteomic Characterization of Ontogenic Changes in Hematopoietic Stem and Progenitor Cells

Jassinskaja, Maria LU ; Johansson, Emil; Kristiansen, Trine Ahn LU ; Åkerstrand, Hugo LU ; Sjöholm, Kristoffer LU ; Hauri, Simon LU ; Malmström, Johan LU ; Yuan, Joan LU and Hansson, Jenny LU (2017) In Cell Reports 21(11). p.3285-3297
Abstract

Hematopoietic stem and progenitor cells (HSPCs) in the fetus and adult possess distinct molecular landscapes that regulate cell fate and change their susceptibility to initiation and progression of hematopoietic malignancies. Here, we applied in-depth quantitative proteomics to comprehensively describe and compare the proteome of fetal and adult HSPCs. Our data uncover a striking difference in complexity of the cellular proteomes, with more diverse adult-specific HSPC proteomic signatures. The differential protein content in fetal and adult HSPCs indicate distinct metabolic profiles and protein complex stoichiometries. Additionally, adult characteristics include an arsenal of proteins linked to viral and bacterial defense, as well as... (More)

Hematopoietic stem and progenitor cells (HSPCs) in the fetus and adult possess distinct molecular landscapes that regulate cell fate and change their susceptibility to initiation and progression of hematopoietic malignancies. Here, we applied in-depth quantitative proteomics to comprehensively describe and compare the proteome of fetal and adult HSPCs. Our data uncover a striking difference in complexity of the cellular proteomes, with more diverse adult-specific HSPC proteomic signatures. The differential protein content in fetal and adult HSPCs indicate distinct metabolic profiles and protein complex stoichiometries. Additionally, adult characteristics include an arsenal of proteins linked to viral and bacterial defense, as well as protection against ROS-induced protein oxidation. Further analyses show that interferon α as well as Neutrophil elastase, has distinct functional effects in fetal and adult HSPCs. This study provides a rich resource aimed toward an enhanced mechanistic understanding of normal and malignant hematopoiesis during fetal and adult life. Jassinskaja et al. describe the proteomic makeup of fetal and adult hematopoietic stem and progenitor cells. They uncover differences in protein complex stoichiometry, defense against pathogens and ROS, and show that IFN-α has distinct functional effects on fetal and adult HSPCs.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
developmental hematopoiesis, hematopoiesis, hematopoietic stem cells, proteomics
in
Cell Reports
volume
21
issue
11
pages
13 pages
publisher
Cell Press
external identifiers
  • scopus:85037839130
  • wos:000417849000024
ISSN
2211-1247
DOI
10.1016/j.celrep.2017.11.070
language
English
LU publication?
yes
id
f03ba5a8-b46d-40b5-9469-52c5482c31a6
date added to LUP
2018-01-03 12:33:47
date last changed
2018-11-21 21:37:00
@article{f03ba5a8-b46d-40b5-9469-52c5482c31a6,
  abstract     = {<p>Hematopoietic stem and progenitor cells (HSPCs) in the fetus and adult possess distinct molecular landscapes that regulate cell fate and change their susceptibility to initiation and progression of hematopoietic malignancies. Here, we applied in-depth quantitative proteomics to comprehensively describe and compare the proteome of fetal and adult HSPCs. Our data uncover a striking difference in complexity of the cellular proteomes, with more diverse adult-specific HSPC proteomic signatures. The differential protein content in fetal and adult HSPCs indicate distinct metabolic profiles and protein complex stoichiometries. Additionally, adult characteristics include an arsenal of proteins linked to viral and bacterial defense, as well as protection against ROS-induced protein oxidation. Further analyses show that interferon α as well as Neutrophil elastase, has distinct functional effects in fetal and adult HSPCs. This study provides a rich resource aimed toward an enhanced mechanistic understanding of normal and malignant hematopoiesis during fetal and adult life. Jassinskaja et al. describe the proteomic makeup of fetal and adult hematopoietic stem and progenitor cells. They uncover differences in protein complex stoichiometry, defense against pathogens and ROS, and show that IFN-α has distinct functional effects on fetal and adult HSPCs.</p>},
  author       = {Jassinskaja, Maria and Johansson, Emil and Kristiansen, Trine Ahn and Åkerstrand, Hugo and Sjöholm, Kristoffer and Hauri, Simon and Malmström, Johan and Yuan, Joan and Hansson, Jenny},
  issn         = {2211-1247},
  keyword      = {developmental hematopoiesis,hematopoiesis,hematopoietic stem cells,proteomics},
  language     = {eng},
  month        = {12},
  number       = {11},
  pages        = {3285--3297},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {Comprehensive Proteomic Characterization of Ontogenic Changes in Hematopoietic Stem and Progenitor Cells},
  url          = {http://dx.doi.org/10.1016/j.celrep.2017.11.070},
  volume       = {21},
  year         = {2017},
}