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Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues.

Krais, Annette LU ; Park, Y.J. ; Reifenberger, G. ; Meister, M. ; Plass, C. and Schmeiser, H. (2019) In Electrophoresis 40(9). p.1293-1297
Abstract
A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Reduced DNA hydroxymethylation was also observed upon dedifferentiation in cultured murine embryonic fibroblasts. Our data show that reduced DNA hydroxymethylation is related to cellular... (More)
A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Reduced DNA hydroxymethylation was also observed upon dedifferentiation in cultured murine embryonic fibroblasts. Our data show that reduced DNA hydroxymethylation is related to cellular dedifferentiation and can be detected in various types of cancers, independent from the IDH1 mutation status. Quantitative determination of altered 5hmC levels may therefore have potential as a biomarker linked to cellular differentiation and tumorigenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
40
issue
9
pages
1293 - 1297
publisher
John Wiley & Sons
external identifiers
  • pmid:30724377
  • scopus:85061614443
ISSN
0173-0835
DOI
10.1002/elps.201800523
language
English
LU publication?
yes
id
f085510d-104a-42c0-9af1-176d13151933
date added to LUP
2019-02-24 22:24:53
date last changed
2020-01-13 01:30:35
@article{f085510d-104a-42c0-9af1-176d13151933,
  abstract     = {A new sensitive analytical method using capillary electrophoresis with laser induced fluorescence (CE-LIF) was applied for the simultaneous detection of DNA methylation and hydroxymethylation levels in human cancers of different origin. DNA hydroxymethylation, measured as 5-hydroxymethylcytosine (5hmC) levels, was decreased in gliomas with mutation in the isocitrate dehydrogenase 1 (IDH1) gene when compared to IDH1-wildtype gliomas. Independent from IDH1 mutation, 5hmC levels were decreased in lung carcinomas when compared to normal lung tissue. Reduced DNA hydroxymethylation was also observed upon dedifferentiation in cultured murine embryonic fibroblasts. Our data show that reduced DNA hydroxymethylation is related to cellular dedifferentiation and can be detected in various types of cancers, independent from the IDH1 mutation status. Quantitative determination of altered 5hmC levels may therefore have potential as a biomarker linked to cellular differentiation and tumorigenesis.},
  author       = {Krais, Annette and Park, Y.J. and Reifenberger, G. and Meister, M. and Plass, C. and Schmeiser, H.},
  issn         = {0173-0835},
  language     = {eng},
  month        = {02},
  number       = {9},
  pages        = {1293--1297},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Sensitive detection of hydroxymethylcytosine levels in normal and neoplastic cells and tissues.},
  url          = {http://dx.doi.org/10.1002/elps.201800523},
  doi          = {10.1002/elps.201800523},
  volume       = {40},
  year         = {2019},
}