Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Exhaled phospholipid transfer protein and hepatocyte growth factor receptor in lung adenocarcinoma

Andreasson, Jesper LU ; Bodén, Embla LU ; Fakhro, Mohammed LU ; von Wachter, Camilla ; Olm, Franziska LU orcid ; Malmsjö, Malin LU ; Hallgren, Oskar LU and Lindstedt, Sandra LU (2022) In Respiratory Research 23(1). p.1-10
Abstract

BACKGROUND: Screening decreases mortality among lung cancer patients but is not widely implemented, thus there is an unmet need for an easily accessible non-invasive method to enable early diagnosis. Particles in exhaled air offer a promising such diagnostic tool. We investigated the validity of a particles in exhaled air device (PExA) to measure the particle flow rate (PFR) and collect exhaled breath particles (EBP) to diagnose primary lung adenocarcinoma (LUAD).

METHODS: Seventeen patients listed for resection of LUAD stages IA-IIIA and 18 non-cancer surgical control patients were enrolled. EBP were collected before and after surgery for LUAD, and once for controls. Proteomic analysis was carried out using a proximity extension... (More)

BACKGROUND: Screening decreases mortality among lung cancer patients but is not widely implemented, thus there is an unmet need for an easily accessible non-invasive method to enable early diagnosis. Particles in exhaled air offer a promising such diagnostic tool. We investigated the validity of a particles in exhaled air device (PExA) to measure the particle flow rate (PFR) and collect exhaled breath particles (EBP) to diagnose primary lung adenocarcinoma (LUAD).

METHODS: Seventeen patients listed for resection of LUAD stages IA-IIIA and 18 non-cancer surgical control patients were enrolled. EBP were collected before and after surgery for LUAD, and once for controls. Proteomic analysis was carried out using a proximity extension assay technology. Results were validated in both plasma from the same cohort and with microarray data from healthy lung tissue and LUAD tissue in the GSE10072 dataset.

RESULTS: Of the 92 proteins analyzed, levels of five proteins in EBP were significantly higher in the LUAD patients compared to controls. Levels of phospholipid transfer protein (PLTP) and hepatocyte growth factor receptor (MET) decreased in LUAD patients after surgery compared to control patients. PFR was significantly higher in the LUAD cohort at all timepoints compared to the control group. MET in plasma correlated significantly with MET in EBP.

CONCLUSION: Collection of EBP and measuring of PFR has never been performed in patients with LUAD. In the present study PFR alone could distinguish between LUAD and patients without LUAD. PLTP and MET were identified as potential biomarkers to evaluate successful tumor excision.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Humans, Phospholipid Transfer Proteins, Proto-Oncogene Proteins c-met, Proteomics, Adenocarcinoma of Lung/diagnosis, Lung Neoplasms/diagnosis, Biomarkers, Tumor/genetics
in
Respiratory Research
volume
23
issue
1
article number
369
pages
1 - 10
publisher
BioMed Central (BMC)
external identifiers
  • scopus:85144557870
  • pmid:36544145
ISSN
1465-9921
DOI
10.1186/s12931-022-02302-4
language
English
LU publication?
yes
additional info
© 2022. The Author(s).
id
f0caef7e-1196-4ea1-9723-76c08645f9c5
date added to LUP
2022-12-26 18:11:54
date last changed
2024-04-18 07:39:42
@article{f0caef7e-1196-4ea1-9723-76c08645f9c5,
  abstract     = {{<p>BACKGROUND: Screening decreases mortality among lung cancer patients but is not widely implemented, thus there is an unmet need for an easily accessible non-invasive method to enable early diagnosis. Particles in exhaled air offer a promising such diagnostic tool. We investigated the validity of a particles in exhaled air device (PExA) to measure the particle flow rate (PFR) and collect exhaled breath particles (EBP) to diagnose primary lung adenocarcinoma (LUAD).</p><p>METHODS: Seventeen patients listed for resection of LUAD stages IA-IIIA and 18 non-cancer surgical control patients were enrolled. EBP were collected before and after surgery for LUAD, and once for controls. Proteomic analysis was carried out using a proximity extension assay technology. Results were validated in both plasma from the same cohort and with microarray data from healthy lung tissue and LUAD tissue in the GSE10072 dataset.</p><p>RESULTS: Of the 92 proteins analyzed, levels of five proteins in EBP were significantly higher in the LUAD patients compared to controls. Levels of phospholipid transfer protein (PLTP) and hepatocyte growth factor receptor (MET) decreased in LUAD patients after surgery compared to control patients. PFR was significantly higher in the LUAD cohort at all timepoints compared to the control group. MET in plasma correlated significantly with MET in EBP.</p><p>CONCLUSION: Collection of EBP and measuring of PFR has never been performed in patients with LUAD. In the present study PFR alone could distinguish between LUAD and patients without LUAD. PLTP and MET were identified as potential biomarkers to evaluate successful tumor excision.</p>}},
  author       = {{Andreasson, Jesper and Bodén, Embla and Fakhro, Mohammed and von Wachter, Camilla and Olm, Franziska and Malmsjö, Malin and Hallgren, Oskar and Lindstedt, Sandra}},
  issn         = {{1465-9921}},
  keywords     = {{Humans; Phospholipid Transfer Proteins; Proto-Oncogene Proteins c-met; Proteomics; Adenocarcinoma of Lung/diagnosis; Lung Neoplasms/diagnosis; Biomarkers, Tumor/genetics}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  pages        = {{1--10}},
  publisher    = {{BioMed Central (BMC)}},
  series       = {{Respiratory Research}},
  title        = {{Exhaled phospholipid transfer protein and hepatocyte growth factor receptor in lung adenocarcinoma}},
  url          = {{http://dx.doi.org/10.1186/s12931-022-02302-4}},
  doi          = {{10.1186/s12931-022-02302-4}},
  volume       = {{23}},
  year         = {{2022}},
}