Use of chemically extracted muscle grafts to repair extended nerve defects in rats
Liu, Xiao-Lin ; Arai, Takeru ; Sondell, Marianne ; Lundborg, Göran LU ; Kanje, Martin LU and Dahlin, Lars LU
(2001)
In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
35(4).
p.337-345
- Abstract
- Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to... (More)
- Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to neovascularisation as showed by staining for endothelial alkaline phosphatase, and limited differences concerning invasion of macrophages (ED1 and ED2) as detected by immunocytochemistry. The results showed that chemically extracted muscle grafts could be used to bridge an extended nerve defect and that such grafts in some aspects were superior to freeze-thawed muscle grafts for extended gaps. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1118522
- author
- Liu, Xiao-Lin
; Arai, Takeru
; Sondell, Marianne
; Lundborg, Göran
LU
; Kanje, Martin
LU
and Dahlin, Lars
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Muscle, Graft, Acellular, Nerve, Regeneration, Schwann, Cell, Macrophages, Immunocytochemistry, Axons, Repair
- in
- Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
- volume
- 35
- issue
- 4
- pages
- 337 - 345
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000173155800001
- scopus:0035663324
- ISSN
- 1651-2073
- DOI
- 10.1080/028443101317149291
- language
- English
- LU publication?
- yes
- id
- f195a240-729d-404c-af84-13cb0b9c08dd (old id 1118522)
- date added to LUP
- 2016-04-01 15:32:13
- date last changed
- 2022-01-28 05:49:05
@article{f195a240-729d-404c-af84-13cb0b9c08dd,
abstract = {{Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to neovascularisation as showed by staining for endothelial alkaline phosphatase, and limited differences concerning invasion of macrophages (ED1 and ED2) as detected by immunocytochemistry. The results showed that chemically extracted muscle grafts could be used to bridge an extended nerve defect and that such grafts in some aspects were superior to freeze-thawed muscle grafts for extended gaps.}},
author = {{Liu, Xiao-Lin and Arai, Takeru and Sondell, Marianne and Lundborg, Göran and Kanje, Martin and Dahlin, Lars}},
issn = {{1651-2073}},
keywords = {{Muscle; Graft; Acellular; Nerve; Regeneration; Schwann; Cell; Macrophages; Immunocytochemistry; Axons; Repair}},
language = {{eng}},
number = {{4}},
pages = {{337--345}},
publisher = {{Taylor & Francis}},
series = {{Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery}},
title = {{Use of chemically extracted muscle grafts to repair extended nerve defects in rats}},
url = {{http://dx.doi.org/10.1080/028443101317149291}},
doi = {{10.1080/028443101317149291}},
volume = {{35}},
year = {{2001}},
}