Evaluation of the prognostic significance of MSMB and CRISP3 in prostate cancer using automated image analysis.
Dahlman, Anna K LU ; Rexhepaj, Elton ; Brennan, Donal J ; Gallagher, William M ; Gaber, Alexander LU ; Lindgren, Anna LU ; Jirström, Karin LU
and Bjartell, Anders
LU
(2011)
In Modern Pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
24.
p.708-719
- Abstract
- Despite prostate cancer being the most frequent cancer in men in the Western world, tissue biomarkers for predicting disease recurrence after surgery have not been incorporated into clinical practice. Our group has previously identified β-microseminoprotein (MSMB) and cysteine-rich secretory protein-3 (CRISP3) as independent predictors of biochemical recurrence after radical prostatectomy. The purpose of the present study was to use automated image analysis, enabling quantitative determination of MSMB and CRISP3 expressions in a large cohort and to validate the previous findings. MSMB and CRISP3 protein expressions were assessed on tissue microarrays constructed from 3268 radical prostatectomy specimens. Whole-slide digital images were... (More)
- Despite prostate cancer being the most frequent cancer in men in the Western world, tissue biomarkers for predicting disease recurrence after surgery have not been incorporated into clinical practice. Our group has previously identified β-microseminoprotein (MSMB) and cysteine-rich secretory protein-3 (CRISP3) as independent predictors of biochemical recurrence after radical prostatectomy. The purpose of the present study was to use automated image analysis, enabling quantitative determination of MSMB and CRISP3 expressions in a large cohort and to validate the previous findings. MSMB and CRISP3 protein expressions were assessed on tissue microarrays constructed from 3268 radical prostatectomy specimens. Whole-slide digital images were captured, and a novel cytoplasmic algorithm was used to develop a quantitative scoring model for cytoplasmic staining. Classification regression tree analysis was used to group patients, with different risk for biochemical recurrence, depending on level of protein expression. Patients with tumors expressing high levels of MSMB had a significantly reduced risk for biochemical recurrence after radical prostatectomy (HR=0.468; 95% CI 0.394-0.556; P<0.001). Multivariate analysis adjusted for clinicopathological parameters revealed that MSMB expression was an independent predictor of decreased risk of recurrence (HR=0.710; 95% CI 0.578-0.872; P<0.001). We found no correlation between CRISP3 expression and biochemical recurrence. In this current study, we applied a novel image analysis on a large independent cohort and successfully verified that MSMB is a strong independent factor, predicting favorable outcome after radical prostatectomy for localized prostate cancer.Modern Pathology advance online publication, 14 January 2011; doi:10.1038/modpathol.2010.238. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1777447
- author
- Dahlman, Anna K
LU
; Rexhepaj, Elton
; Brennan, Donal J
; Gallagher, William M
; Gaber, Alexander
LU
; Lindgren, Anna
LU
; Jirström, Karin
LU
and Bjartell, Anders
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Modern Pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
- volume
- 24
- pages
- 708 - 719
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000290091900011
- pmid:21240253
- scopus:79955574256
- pmid:21240253
- ISSN
- 1530-0285
- DOI
- 10.1038/modpathol.2010.238
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Urological Cancers (013243420), Pathology (Malmö) (013031000), Pathology, (Lund) (013030000)
- id
- f267283d-26dd-492f-be16-f17e4731d079 (old id 1777447)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21240253?dopt=Abstract
- date added to LUP
- 2016-04-01 13:34:52
- date last changed
- 2024-02-25 00:44:38
@article{f267283d-26dd-492f-be16-f17e4731d079,
abstract = {{Despite prostate cancer being the most frequent cancer in men in the Western world, tissue biomarkers for predicting disease recurrence after surgery have not been incorporated into clinical practice. Our group has previously identified β-microseminoprotein (MSMB) and cysteine-rich secretory protein-3 (CRISP3) as independent predictors of biochemical recurrence after radical prostatectomy. The purpose of the present study was to use automated image analysis, enabling quantitative determination of MSMB and CRISP3 expressions in a large cohort and to validate the previous findings. MSMB and CRISP3 protein expressions were assessed on tissue microarrays constructed from 3268 radical prostatectomy specimens. Whole-slide digital images were captured, and a novel cytoplasmic algorithm was used to develop a quantitative scoring model for cytoplasmic staining. Classification regression tree analysis was used to group patients, with different risk for biochemical recurrence, depending on level of protein expression. Patients with tumors expressing high levels of MSMB had a significantly reduced risk for biochemical recurrence after radical prostatectomy (HR=0.468; 95% CI 0.394-0.556; P<0.001). Multivariate analysis adjusted for clinicopathological parameters revealed that MSMB expression was an independent predictor of decreased risk of recurrence (HR=0.710; 95% CI 0.578-0.872; P<0.001). We found no correlation between CRISP3 expression and biochemical recurrence. In this current study, we applied a novel image analysis on a large independent cohort and successfully verified that MSMB is a strong independent factor, predicting favorable outcome after radical prostatectomy for localized prostate cancer.Modern Pathology advance online publication, 14 January 2011; doi:10.1038/modpathol.2010.238.}},
author = {{Dahlman, Anna K and Rexhepaj, Elton and Brennan, Donal J and Gallagher, William M and Gaber, Alexander and Lindgren, Anna and Jirström, Karin and Bjartell, Anders}},
issn = {{1530-0285}},
language = {{eng}},
pages = {{708--719}},
publisher = {{Nature Publishing Group}},
series = {{Modern Pathology : an official journal of the United States and Canadian Academy of Pathology, Inc}},
title = {{Evaluation of the prognostic significance of MSMB and CRISP3 in prostate cancer using automated image analysis.}},
url = {{https://lup.lub.lu.se/search/files/3457423/1848354.pdf}},
doi = {{10.1038/modpathol.2010.238}},
volume = {{24}},
year = {{2011}},
}