Quantification method of human hemoglobin adducts from hexahydrophthalic anhydride and methylhexahydrophthalic anhydride
(1998) In Journal of Chromatography. B 710(1-2). p.81-90- Abstract
- A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb... (More)
- A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb for MHHP acid. The between-day precisions for HHP acid were 18% at 2 pmol/g Hb and 10% at 13 pmol/g Hb. For MHHP acid, the precision was 27% at 2 pmol/g Hb and 14% at 22 pmol/g Hb. The method was applicable for analysis of Hb adducts from workers occupationally exposed to HHPA and MHHPA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113365
- author
- Lindh, Christian LU and Jönsson, Bo A LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hexahydrophthalic anhydride, Hemogloblin, Methylhexahydrophthalic anhydride
- in
- Journal of Chromatography. B
- volume
- 710
- issue
- 1-2
- pages
- 81 - 90
- publisher
- Elsevier
- external identifiers
-
- pmid:9686874
- scopus:0032511242
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(98)00121-2
- language
- English
- LU publication?
- yes
- id
- f5179cef-8026-448b-b821-8dde7ee3f36e (old id 1113365)
- date added to LUP
- 2016-04-01 16:40:55
- date last changed
- 2022-01-28 21:23:09
@article{f5179cef-8026-448b-b821-8dde7ee3f36e, abstract = {{A method was developed for the determination of human hemoglobin (Hb) adducts from hexahydrophthalic anhydride (HHPA) and methylhexahydrophthalic anhydride (MHHPA). The procedure includes lysis of erythrocytes, dialysis of the Hb-solution followed by acid hydrolysis. The released hexahydrophthalic (HHP) acid and methylhexahydrophthalic (MHHP) acid were purified using a combined liquid-liquid and solid-phase extraction procedure followed by derivatization with pentafluorobenzyl bromide. The derivatives were analyzed using GC-MS in negative ion chemical ionization mode with ammonia as moderating gas. As internal standards, deuterium-labeled HHP and MHHP acids were used. The detection limits were 0.3 pmol/g Hb for HHP acid and 0.9 pmol/g Hb for MHHP acid. The between-day precisions for HHP acid were 18% at 2 pmol/g Hb and 10% at 13 pmol/g Hb. For MHHP acid, the precision was 27% at 2 pmol/g Hb and 14% at 22 pmol/g Hb. The method was applicable for analysis of Hb adducts from workers occupationally exposed to HHPA and MHHPA.}}, author = {{Lindh, Christian and Jönsson, Bo A}}, issn = {{1387-2273}}, keywords = {{Hexahydrophthalic anhydride; Hemogloblin; Methylhexahydrophthalic anhydride}}, language = {{eng}}, number = {{1-2}}, pages = {{81--90}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Quantification method of human hemoglobin adducts from hexahydrophthalic anhydride and methylhexahydrophthalic anhydride}}, url = {{http://dx.doi.org/10.1016/S0378-4347(98)00121-2}}, doi = {{10.1016/S0378-4347(98)00121-2}}, volume = {{710}}, year = {{1998}}, }