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Counter-diffusion studies of human transthyretin : the growth of high-quality crystals for X-ray and neutron crystallography

De'ath, Clare ; Oliva, Mizar F. ; Moulin, Martine ; Blakeley, Matthew P. ; Haertlein, Michael ; Mitchell, Edward P. ; Gavira, José A. ; Bowler, Matthew W. and Forsyth, V. Trevor LU (2025) In Journal of Applied Crystallography 58(Pt 1). p.107-118
Abstract

A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amyloidogenesis, counter-diffusion crystallization, crystallogenesis, large-volume crystals, macromolecular deuteration, neutron macromolecular crystallography, perdeuteration, transthyretin, X-ray diffraction
in
Journal of Applied Crystallography
volume
58
issue
Pt 1
pages
12 pages
publisher
International Union of Crystallography
external identifiers
  • scopus:85217931116
ISSN
0021-8898
DOI
10.1107/S1600576724011191
language
English
LU publication?
yes
id
f569170c-1b7e-4481-bbe4-4e6b2020d5e3
date added to LUP
2025-06-25 10:09:38
date last changed
2025-06-25 10:09:54
@article{f569170c-1b7e-4481-bbe4-4e6b2020d5e3,
  abstract     = {{<p>A crystallogenesis study of human transthyretin using the counter-diffusion method is described as an alternative to conventional convective vapour diffusion and batch approaches for protein crystallization. The X-ray diffraction results show systematic trends that exhibit unique patterns of crystallization and high crystal quality as well as a remarkable degree of coherence within extended crystal rods that wholly fill the capillaries used. Preliminary neutron diffraction data have been recorded from a number of these samples, validating the feasibility of this methodology for neutron crystallography.</p>}},
  author       = {{De'ath, Clare and Oliva, Mizar F. and Moulin, Martine and Blakeley, Matthew P. and Haertlein, Michael and Mitchell, Edward P. and Gavira, José A. and Bowler, Matthew W. and Forsyth, V. Trevor}},
  issn         = {{0021-8898}},
  keywords     = {{amyloidogenesis; counter-diffusion crystallization; crystallogenesis; large-volume crystals; macromolecular deuteration; neutron macromolecular crystallography; perdeuteration; transthyretin; X-ray diffraction}},
  language     = {{eng}},
  number       = {{Pt 1}},
  pages        = {{107--118}},
  publisher    = {{International Union of Crystallography}},
  series       = {{Journal of Applied Crystallography}},
  title        = {{Counter-diffusion studies of human transthyretin : the growth of high-quality crystals for X-ray and neutron crystallography}},
  url          = {{http://dx.doi.org/10.1107/S1600576724011191}},
  doi          = {{10.1107/S1600576724011191}},
  volume       = {{58}},
  year         = {{2025}},
}