In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.
Ekblad, Lars LU
; Baldetorp, Bo
LU
; Fernö, Mårten
LU
; Olsson, Håkan
LU
and Welinder, Charlotte
LU
(2007)
In Journal of Proteome Research
6(4).
p.1609-1614
- Abstract
- SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/166341
- author
- Ekblad, Lars
LU
; Baldetorp, Bo
LU
; Fernö, Mårten
LU
; Olsson, Håkan
LU
and Welinder, Charlotte
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Proteome Research
- volume
- 6
- issue
- 4
- pages
- 1609 - 1614
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000245510900038
- scopus:34248221112
- ISSN
- 1535-3893
- DOI
- 10.1021/pr060633y
- language
- English
- LU publication?
- yes
- id
- f5b8d001-c8ca-4cbf-8fc7-dec205e325e6 (old id 166341)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17373843&dopt=Abstract
- date added to LUP
- 2016-04-01 12:30:06
- date last changed
- 2022-01-27 05:54:20
@article{f5b8d001-c8ca-4cbf-8fc7-dec205e325e6,
abstract = {{SELDI-TOF MS is a mass spectrometric technique which has been extensively used for biomarker discovery. In this study, we show that in-source decay is an important source for the generation of additional spectral peaks with this technique, both for pure proteins and proteins in serum samples. Thus, SELDI-TOF MS could be used to gain sequence information from proteins, but the results also question the uncritical use of SELDI-TOF MS as a general method for the detection of biomarkers.}},
author = {{Ekblad, Lars and Baldetorp, Bo and Fernö, Mårten and Olsson, Håkan and Welinder, Charlotte}},
issn = {{1535-3893}},
language = {{eng}},
number = {{4}},
pages = {{1609--1614}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of Proteome Research}},
title = {{In-Source Decay Causes Artifacts in SELDI-TOF MS Spectra.}},
url = {{http://dx.doi.org/10.1021/pr060633y}},
doi = {{10.1021/pr060633y}},
volume = {{6}},
year = {{2007}},
}