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Fluorescence lifetime imaging through scattering media

Nilsson, Sebastian LU ; Kristensson, Elias LU ; Aldén, Marcus LU ; Bood, Joakim LU and Ehn, Andreas LU (2023) In Scientific Reports
Abstract
Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are being mixed, resulting in erroneous results. Such issues occur when the contrast of the measurement object is low, which can be limiting in applied measurements due to spurious light scattering. A solution is presented here where structured illumination is used to enhance image contrast in fluorescence lifetime wide-field imaging. Lifetime imaging determination was carried out using Dual Imaging Modeling Evaluation (DIME), and spatial lock-in analysis... (More)
Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are being mixed, resulting in erroneous results. Such issues occur when the contrast of the measurement object is low, which can be limiting in applied measurements due to spurious light scattering. A solution is presented here where structured illumination is used to enhance image contrast in fluorescence lifetime wide-field imaging. Lifetime imaging determination was carried out using Dual Imaging Modeling Evaluation (DIME), and spatial lock-in analysis was used for removing spurious scattered signal to enable fluorescence lifetime imaging through scattering media. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
article number
3066
publisher
Nature Publishing Group
external identifiers
  • scopus:85148548054
  • pmid:36810512
ISSN
2045-2322
DOI
10.1038/s41598-023-30055-7
project
HYdrogen as a FLEXible energy storage for a fully renewable European POWER system
Advanced Laser Diagnostics for Discharge Plasma
language
English
LU publication?
yes
id
f64397cb-b432-4aaa-83c5-a19ed99b9d63
date added to LUP
2023-02-28 10:04:08
date last changed
2023-11-06 18:02:35
@article{f64397cb-b432-4aaa-83c5-a19ed99b9d63,
  abstract     = {{Fluorescence lifetime determination has proven to be useful, e.g. identification of molecules, quantitative estimation of species concentration and determination of temperatures. Lifetime determination of exponentially decaying signals is challenging if signals of different decay rates are being mixed, resulting in erroneous results. Such issues occur when the contrast of the measurement object is low, which can be limiting in applied measurements due to spurious light scattering. A solution is presented here where structured illumination is used to enhance image contrast in fluorescence lifetime wide-field imaging. Lifetime imaging determination was carried out using Dual Imaging Modeling Evaluation (DIME), and spatial lock-in analysis was used for removing spurious scattered signal to enable fluorescence lifetime imaging through scattering media.}},
  author       = {{Nilsson, Sebastian and Kristensson, Elias and Aldén, Marcus and Bood, Joakim and Ehn, Andreas}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{02}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Fluorescence lifetime imaging through scattering media}},
  url          = {{http://dx.doi.org/10.1038/s41598-023-30055-7}},
  doi          = {{10.1038/s41598-023-30055-7}},
  year         = {{2023}},
}