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Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study

Myhrstad, Mari C.W. ; de Mello, Vanessa D. ; Dahlman, Ingrid ; Kolehmainen, Marjukka ; Paananen, Jussi ; Rundblad, Amanda ; Carlberg, Carsten ; Olstad, Ole Kristoffer ; Pihlajamäki, Jussi and Holven, Kirsten B. , et al. (2019) In Molecular Nutrition and Food Research 63(13).
Abstract

Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in... (More)

Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

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publication status
published
subject
keywords
gene-expression, healthy Nordic diet, metabolic syndrome, peripheral blood mononuclear cells, transcriptome
in
Molecular Nutrition and Food Research
volume
63
issue
13
article number
1801405
publisher
John Wiley and Sons Inc.
external identifiers
  • pmid:30964598
  • scopus:85064710489
ISSN
1613-4125
DOI
10.1002/mnfr.201801405
language
English
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yes
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f6d3e570-d49e-4af5-ad21-ad4f26e42c5a
date added to LUP
2019-05-07 14:17:10
date last changed
2020-08-12 08:37:08
@article{f6d3e570-d49e-4af5-ad21-ad4f26e42c5a,
  abstract     = {<p>Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value &lt; 0.05). Twenty-five of these are significantly regulated (FDR q-value &lt; 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.</p>},
  author       = {Myhrstad, Mari C.W. and de Mello, Vanessa D. and Dahlman, Ingrid and Kolehmainen, Marjukka and Paananen, Jussi and Rundblad, Amanda and Carlberg, Carsten and Olstad, Ole Kristoffer and Pihlajamäki, Jussi and Holven, Kirsten B. and Hermansen, Kjeld and Dragsted, Lars O. and Gunnarsdottir, Ingibjörg and Cloetens, Lieselotte and Storm, Matilda Ulmius and Åkesson, Björn and Rosqvist, Fredrik and Hukkanen, Janne and Herzig, Karl Heinz and Risérus, Ulf and Thorsdottir, Inga and Poutanen, Kaisa S. and Savolainen, Markku J. and Schwab, Ursula and Arner, Peter and Uusitupa, Matti and Ulven, Stine M.},
  issn         = {1613-4125},
  language     = {eng},
  number       = {13},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Molecular Nutrition and Food Research},
  title        = {Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study},
  url          = {http://dx.doi.org/10.1002/mnfr.201801405},
  doi          = {10.1002/mnfr.201801405},
  volume       = {63},
  year         = {2019},
}