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Axonal outgrowth on nano-imprinted patterns

Johansson, Fredrik I LU ; Carlberg, Patrick LU ; Danielsen, Nils LU ; Montelius, Lars LU and Kanje, Martin LU (2006) In Biomaterials 27(8). p.1251-1258
Abstract
Nanotechnology has provided methods to fabricate surface patterns with features down to a few rim. If cells or cell processes exhibit contact guidance in response to such small patterns is an interesting question and could be pertinent for many applications. In the present study we investigated if axonal outgrowth was affected by nano-printed patterns in polymethylmethacrylate (PMMA)-covered silicon chips. To this end adult mouse sympathetic and sensory ganglia were mounted in Matrigel (R) on the chips close to the nano-patterns. The patterns consisted of parallel grooves with depths of 300 nm and varying widths of 100-400 nm. The distance between two adjacent grooves was 100-1600 nm. The chips were cultured in medium containing 25 ng/ml... (More)
Nanotechnology has provided methods to fabricate surface patterns with features down to a few rim. If cells or cell processes exhibit contact guidance in response to such small patterns is an interesting question and could be pertinent for many applications. In the present study we investigated if axonal outgrowth was affected by nano-printed patterns in polymethylmethacrylate (PMMA)-covered silicon chips. To this end adult mouse sympathetic and sensory ganglia were mounted in Matrigel (R) on the chips close to the nano-patterns. The patterns consisted of parallel grooves with depths of 300 nm and varying widths of 100-400 nm. The distance between two adjacent grooves was 100-1600 nm. The chips were cultured in medium containing 25 ng/ml of nerve growth factor to stimulate axonal outgrowth. After 1 week of incubation. axonal outgrowth was investigated by immunocytochemistry or scanning electron microscopy. Axons displayed contact guidance on all patterns. Furthermore, we found that the nerve cell processes preferred to grow on ridge edges and elevations in the patterns rather than in grooves, a seemingly claustrophobic behavior. We conclude that axons of peripheral neurons might be guided by nanopatterns on PMMA when the lateral features are 100 nm or larger. The present results can be utilized for nerve regenerating scaffolds or the construction of a stable, high-resolution electronic interface to neurons, which is required for future brain machine interfaces. (c) 2005 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nanotopography, nerve regeneration, nerve guide, cell culture, polymethylmethacrylate
in
Biomaterials
volume
27
issue
8
pages
1251 - 1258
publisher
Elsevier
external identifiers
  • wos:000234731900008
  • pmid:16143385
  • scopus:28444479047
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2005.07.047
language
English
LU publication?
yes
id
f8303cbc-ac4a-4f7c-a858-b53ebdc4aa9a (old id 419959)
date added to LUP
2007-10-05 15:40:33
date last changed
2019-08-14 01:53:54
@article{f8303cbc-ac4a-4f7c-a858-b53ebdc4aa9a,
  abstract     = {Nanotechnology has provided methods to fabricate surface patterns with features down to a few rim. If cells or cell processes exhibit contact guidance in response to such small patterns is an interesting question and could be pertinent for many applications. In the present study we investigated if axonal outgrowth was affected by nano-printed patterns in polymethylmethacrylate (PMMA)-covered silicon chips. To this end adult mouse sympathetic and sensory ganglia were mounted in Matrigel (R) on the chips close to the nano-patterns. The patterns consisted of parallel grooves with depths of 300 nm and varying widths of 100-400 nm. The distance between two adjacent grooves was 100-1600 nm. The chips were cultured in medium containing 25 ng/ml of nerve growth factor to stimulate axonal outgrowth. After 1 week of incubation. axonal outgrowth was investigated by immunocytochemistry or scanning electron microscopy. Axons displayed contact guidance on all patterns. Furthermore, we found that the nerve cell processes preferred to grow on ridge edges and elevations in the patterns rather than in grooves, a seemingly claustrophobic behavior. We conclude that axons of peripheral neurons might be guided by nanopatterns on PMMA when the lateral features are 100 nm or larger. The present results can be utilized for nerve regenerating scaffolds or the construction of a stable, high-resolution electronic interface to neurons, which is required for future brain machine interfaces. (c) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Johansson, Fredrik I and Carlberg, Patrick and Danielsen, Nils and Montelius, Lars and Kanje, Martin},
  issn         = {1878-5905},
  keyword      = {nanotopography,nerve regeneration,nerve guide,cell culture,polymethylmethacrylate},
  language     = {eng},
  number       = {8},
  pages        = {1251--1258},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Axonal outgrowth on nano-imprinted patterns},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2005.07.047},
  volume       = {27},
  year         = {2006},
}