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Occupational second-hand smoke exposure : A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers

Neves, Sofia ; Pacheco, Solange LU ; Vaz, Fátima ; James, Peter LU orcid ; Simões, Tânia and Penque, Deborah (2024) In Environmental Toxicology and Pharmacology 108.
Abstract

Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in... (More)

Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cigarette smoke, Mass Spectrometry, Nasal epithelium, Protein network, Proteomics, Second-Hand Smoke
in
Environmental Toxicology and Pharmacology
volume
108
article number
104459
publisher
Elsevier
external identifiers
  • pmid:38685369
  • scopus:85192172558
ISSN
1382-6689
DOI
10.1016/j.etap.2024.104459
language
English
LU publication?
yes
id
f9af0c3c-9ffe-44ac-a5a7-1aabfa26516f
date added to LUP
2024-05-16 15:47:13
date last changed
2024-05-30 17:38:29
@article{f9af0c3c-9ffe-44ac-a5a7-1aabfa26516f,
  abstract     = {{<p>Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.</p>}},
  author       = {{Neves, Sofia and Pacheco, Solange and Vaz, Fátima and James, Peter and Simões, Tânia and Penque, Deborah}},
  issn         = {{1382-6689}},
  keywords     = {{Cigarette smoke; Mass Spectrometry; Nasal epithelium; Protein network; Proteomics; Second-Hand Smoke}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Environmental Toxicology and Pharmacology}},
  title        = {{Occupational second-hand smoke exposure : A comparative shotgun proteomics study on nasal epithelia from healthy restaurant workers}},
  url          = {{http://dx.doi.org/10.1016/j.etap.2024.104459}},
  doi          = {{10.1016/j.etap.2024.104459}},
  volume       = {{108}},
  year         = {{2024}},
}