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The adsorption of lysozyme to hydrophilic silicon oxide surfaces: comparison between experimental data and models for adsorption kinetics

Wahlgren, Marie LU ; Arnebrant, Thomas and Lundström, Ingemar LU (1995) In Journal of Colloid and Interface Science 175(2). p.506-514
Abstract
The adsorption of lysozyme onto silicon oxide surfaces was investigated using in situ ellipsometry. Both the adsorption kinetics and the desorption upon rinsing with buffer were followed and the experimental data obtained were compared to models for adsorption kinetics. The effects of concentration, time of adsorption, and ionic strength were studied. The adsorbed amount reached a semiplateau within an hour of adsorption and only partial desorption was observed upon rinsing. The adsorption was found to be affected by the ionic strength and the following parameters were seen to increase in buffers of low ionic strength; the amount adsorbed, the rate of initial adsorption, and the amount adsorbed irreversibly after rinsing with buffer. The... (More)
The adsorption of lysozyme onto silicon oxide surfaces was investigated using in situ ellipsometry. Both the adsorption kinetics and the desorption upon rinsing with buffer were followed and the experimental data obtained were compared to models for adsorption kinetics. The effects of concentration, time of adsorption, and ionic strength were studied. The adsorbed amount reached a semiplateau within an hour of adsorption and only partial desorption was observed upon rinsing. The adsorption was found to be affected by the ionic strength and the following parameters were seen to increase in buffers of low ionic strength; the amount adsorbed, the rate of initial adsorption, and the amount adsorbed irreversibly after rinsing with buffer. The amount adsorbed after 1 h did not reach a plateau within the concentration range investigated, in spite of reaching levels above monolayer coverage. The fraction adsorbed irreversibly increased during the time interval studied (1 h). The experimental data could be fitted to a model involving lysozyme adsorbed in three states. These include molecules adsorbed reversibly to the surface, models adsorbed irreversibly to the surface, and molecules adsorbed in a second layer. The second layer was considered to adsorb irreversibly to the molecules in the first layer. Furthermore, the rate constants were dependent on the surface coverage and the model allowed for exchange between adsorbed molecules and those in solution. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Colloid and Interface Science
volume
175
issue
2
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:0001658553
ISSN
0021-9797
DOI
10.1006/jcis.1995.1482
language
English
LU publication?
yes
id
fbba3e42-11b2-486d-9e64-5a6443208305
date added to LUP
2016-04-15 19:37:04
date last changed
2017-08-27 06:15:31
@article{fbba3e42-11b2-486d-9e64-5a6443208305,
  abstract     = {The adsorption of lysozyme onto silicon oxide surfaces was investigated using in situ ellipsometry. Both the adsorption kinetics and the desorption upon rinsing with buffer were followed and the experimental data obtained were compared to models for adsorption kinetics. The effects of concentration, time of adsorption, and ionic strength were studied. The adsorbed amount reached a semiplateau within an hour of adsorption and only partial desorption was observed upon rinsing. The adsorption was found to be affected by the ionic strength and the following parameters were seen to increase in buffers of low ionic strength; the amount adsorbed, the rate of initial adsorption, and the amount adsorbed irreversibly after rinsing with buffer. The amount adsorbed after 1 h did not reach a plateau within the concentration range investigated, in spite of reaching levels above monolayer coverage. The fraction adsorbed irreversibly increased during the time interval studied (1 h). The experimental data could be fitted to a model involving lysozyme adsorbed in three states. These include molecules adsorbed reversibly to the surface, models adsorbed irreversibly to the surface, and molecules adsorbed in a second layer. The second layer was considered to adsorb irreversibly to the molecules in the first layer. Furthermore, the rate constants were dependent on the surface coverage and the model allowed for exchange between adsorbed molecules and those in solution.},
  author       = {Wahlgren, Marie and Arnebrant, Thomas and Lundström, Ingemar},
  issn         = {0021-9797},
  language     = {eng},
  number       = {2},
  pages        = {506--514},
  publisher    = {Elsevier},
  series       = {Journal of Colloid and Interface Science},
  title        = {The adsorption of lysozyme to hydrophilic silicon oxide surfaces: comparison between experimental data and models for adsorption kinetics},
  url          = {http://dx.doi.org/10.1006/jcis.1995.1482},
  volume       = {175},
  year         = {1995},
}