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Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection

Lindh, Christian LU orcid and Jönsson, Bo A LU (1997) In Journal of Chromatography. B 691(2). p.331-339
Abstract
A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall... (More)
A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers. (Less)
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Contribution to journal
publication status
published
subject
keywords
Hexahydrophthalic acid, Methylhexahydrophthalic acid
in
Journal of Chromatography. B
volume
691
issue
2
pages
331 - 339
publisher
Elsevier
external identifiers
  • pmid:9174269
  • scopus:0343118748
ISSN
1387-2273
DOI
10.1016/S0378-4347(96)00479-3
language
English
LU publication?
yes
id
fcaeae8a-4053-48e9-b9c8-2eff5415593c (old id 1111497)
date added to LUP
2016-04-01 16:41:51
date last changed
2022-01-28 21:28:37
@article{fcaeae8a-4053-48e9-b9c8-2eff5415593c,
  abstract     = {{A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers.}},
  author       = {{Lindh, Christian and Jönsson, Bo A}},
  issn         = {{1387-2273}},
  keywords     = {{Hexahydrophthalic acid; Methylhexahydrophthalic acid}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{331--339}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection}},
  url          = {{http://dx.doi.org/10.1016/S0378-4347(96)00479-3}},
  doi          = {{10.1016/S0378-4347(96)00479-3}},
  volume       = {{691}},
  year         = {{1997}},
}