Advanced

MMP7 modulation by short- and long-term radiotherapy in patients with rectal cancer

Stene, Christina LU ; Polistena, Andrea LU ; Gaber, Alexander LU ; Nodin, Björn LU ; Ottochian, Bianca; Adawi, Diya LU ; Avenia, Nicola; Jirström, Karin LU and Johnson, Louis Banka LU (2018) In In Vivo 32(1). p.133-138
Abstract

Background/Aim: Matrix metalloproteinase 7 (MMP7) expression is highly associated with colorectal cancer and modulates tumour growth and invasion. Radiation injury induces inflammation with increases in MMP7 and in transforming growth factor beta (TGFβ). The aim of this study was to investigate the effect on MMP7 and TGFβ. expression in patients with rectal cancer undergoing different regimens of neoadjuvant radiotherapy (RT). Patients and Methods: We studied 53 patients in three RT treatment groups receiving RT of 25 Gy, long-term RT 50 Gy and controls receiving no RT. Three biopsies were obtained from each patient during the treatments: before RT, after RT and after surgery. Tissue samples were formalin fixed, paraffin embedded and... (More)

Background/Aim: Matrix metalloproteinase 7 (MMP7) expression is highly associated with colorectal cancer and modulates tumour growth and invasion. Radiation injury induces inflammation with increases in MMP7 and in transforming growth factor beta (TGFβ). The aim of this study was to investigate the effect on MMP7 and TGFβ. expression in patients with rectal cancer undergoing different regimens of neoadjuvant radiotherapy (RT). Patients and Methods: We studied 53 patients in three RT treatment groups receiving RT of 25 Gy, long-term RT 50 Gy and controls receiving no RT. Three biopsies were obtained from each patient during the treatments: before RT, after RT and after surgery. Tissue samples were formalin fixed, paraffin embedded and tissue microarrays were constructed and stained for MMP7 and TGFβ. Mann–Whitney U-tests and Wilcoxon Z-tests were used to determine differences between patients before and after RT, and after surgery, as well as between the RT groups. Results: In all three patient groups, increases of MMP7 and TGFβ expression were observed after surgery. MMP7 expression was significantly increased in patients receiving short-term RT but TGFβ expression was not affected by RT. Conclusion: 50 Gy Irradiation of rectal cancer gives less tumour activation of MMP7, whilst it is up-regulated by 25 Gy and surgery regardless of RT.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Inflammation, Matrilysin, Matrix metalloproteinases, Radiotherapy, Rectal cancer
in
In Vivo
volume
32
issue
1
pages
6 pages
publisher
In vivo
external identifiers
  • scopus:85039710736
ISSN
0258-851X
DOI
10.21873/invivo.11215
language
English
LU publication?
yes
id
fd953b6c-3639-4293-aee0-fefd9643fd53
date added to LUP
2018-01-09 10:40:01
date last changed
2018-07-11 03:00:40
@article{fd953b6c-3639-4293-aee0-fefd9643fd53,
  abstract     = {<p>Background/Aim: Matrix metalloproteinase 7 (MMP7) expression is highly associated with colorectal cancer and modulates tumour growth and invasion. Radiation injury induces inflammation with increases in MMP7 and in transforming growth factor beta (TGFβ). The aim of this study was to investigate the effect on MMP7 and TGFβ. expression in patients with rectal cancer undergoing different regimens of neoadjuvant radiotherapy (RT). Patients and Methods: We studied 53 patients in three RT treatment groups receiving RT of 25 Gy, long-term RT 50 Gy and controls receiving no RT. Three biopsies were obtained from each patient during the treatments: before RT, after RT and after surgery. Tissue samples were formalin fixed, paraffin embedded and tissue microarrays were constructed and stained for MMP7 and TGFβ. Mann–Whitney U-tests and Wilcoxon Z-tests were used to determine differences between patients before and after RT, and after surgery, as well as between the RT groups. Results: In all three patient groups, increases of MMP7 and TGFβ expression were observed after surgery. MMP7 expression was significantly increased in patients receiving short-term RT but TGFβ expression was not affected by RT. Conclusion: 50 Gy Irradiation of rectal cancer gives less tumour activation of MMP7, whilst it is up-regulated by 25 Gy and surgery regardless of RT.</p>},
  author       = {Stene, Christina and Polistena, Andrea and Gaber, Alexander and Nodin, Björn and Ottochian, Bianca and Adawi, Diya and Avenia, Nicola and Jirström, Karin and Johnson, Louis Banka},
  issn         = {0258-851X},
  keyword      = {Inflammation,Matrilysin,Matrix metalloproteinases,Radiotherapy,Rectal cancer},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {133--138},
  publisher    = {In vivo},
  series       = {In Vivo},
  title        = {MMP7 modulation by short- and long-term radiotherapy in patients with rectal cancer},
  url          = {http://dx.doi.org/10.21873/invivo.11215},
  volume       = {32},
  year         = {2018},
}