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Gradient acoustic focusing of sub-micron particles for separation of bacteria from blood lysate

Van Assche, David LU ; Reithuber, Elisabeth ; Qiu, Wei LU orcid ; Laurell, Thomas LU ; Henriques-Normark, Birgitta ; Mellroth, Peter ; Ohlsson, Pelle LU orcid and Augustsson, Per LU (2020) In Scientific Reports 10(1).
Abstract

Handling of submicron-sized objects is important in many biochemical and biomedical applications, but few methods today can precisely manipulate this range of particles. We present gradient acoustic focusing that enables flow-through particle separation of submicron particles and cells and we apply it for separation of bacteria from blood lysate to facilitate their detection in whole blood for improved diagnostics. To control suspended objects below the classical 2µm size limit for acoustic focusing, we introduce a co-flowing acoustic impedance gradient to generate a stabilizing acoustic volume force that supresses acoustic streaming. The method is validated theoretically and experimentally using polystyrene particles, Staphylococcus... (More)

Handling of submicron-sized objects is important in many biochemical and biomedical applications, but few methods today can precisely manipulate this range of particles. We present gradient acoustic focusing that enables flow-through particle separation of submicron particles and cells and we apply it for separation of bacteria from blood lysate to facilitate their detection in whole blood for improved diagnostics. To control suspended objects below the classical 2µm size limit for acoustic focusing, we introduce a co-flowing acoustic impedance gradient to generate a stabilizing acoustic volume force that supresses acoustic streaming. The method is validated theoretically and experimentally using polystyrene particles, Staphylococcus aureus, Streptococcus pneumoniae and Escherichia coli. The applicability of the method is demonstrated by the separation of bacteria from selectively chemically lysed blood. Combined with downstream operations, this new approach opens up for novel methods for sepsis diagnostics.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bacterial infection, Biomedical engineering, Fluid dynamics
in
Scientific Reports
volume
10
issue
1
article number
3670
publisher
Nature Publishing Group
external identifiers
  • pmid:32111864
  • scopus:85080840081
ISSN
2045-2322
DOI
10.1038/s41598-020-60338-2
language
English
LU publication?
yes
id
fe4d2178-9648-46f0-9f34-11280d9a1d19
date added to LUP
2020-03-09 14:09:05
date last changed
2024-05-30 11:55:47
@article{fe4d2178-9648-46f0-9f34-11280d9a1d19,
  abstract     = {{<p>Handling of submicron-sized objects is important in many biochemical and biomedical applications, but few methods today can precisely manipulate this range of particles. We present gradient acoustic focusing that enables flow-through particle separation of submicron particles and cells and we apply it for separation of bacteria from blood lysate to facilitate their detection in whole blood for improved diagnostics. To control suspended objects below the classical 2µm size limit for acoustic focusing, we introduce a co-flowing acoustic impedance gradient to generate a stabilizing acoustic volume force that supresses acoustic streaming. The method is validated theoretically and experimentally using polystyrene particles, Staphylococcus aureus, Streptococcus pneumoniae and Escherichia coli. The applicability of the method is demonstrated by the separation of bacteria from selectively chemically lysed blood. Combined with downstream operations, this new approach opens up for novel methods for sepsis diagnostics.</p>}},
  author       = {{Van Assche, David and Reithuber, Elisabeth and Qiu, Wei and Laurell, Thomas and Henriques-Normark, Birgitta and Mellroth, Peter and Ohlsson, Pelle and Augustsson, Per}},
  issn         = {{2045-2322}},
  keywords     = {{Bacterial infection; Biomedical engineering; Fluid dynamics}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Gradient acoustic focusing of sub-micron particles for separation of bacteria from blood lysate}},
  url          = {{http://dx.doi.org/10.1038/s41598-020-60338-2}},
  doi          = {{10.1038/s41598-020-60338-2}},
  volume       = {{10}},
  year         = {{2020}},
}