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Purification and crystallization of the I subunit of magnesium chelatase

Lv, You LU (2011) KEMT10 20111
Department of Chemistry
Abstract
The I subunit of magnesium chelatase is an AAA+ protein which binds and
hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.
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author
Lv, You LU
supervisor
organization
course
KEMT10 20111
year
type
H2 - Master's Degree (Two Years)
subject
keywords
Magnesium chelatase, BchI, ChlI, AAA+ protein, Proteinvetenskap
language
English
id
3053630
date added to LUP
2012-09-19 11:48:11
date last changed
2012-09-19 11:48:11
@misc{3053630,
  abstract     = {The I subunit of magnesium chelatase is an AAA+ protein which binds and
hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.},
  author       = {Lv, You},
  keyword      = {Magnesium chelatase,BchI,ChlI,AAA+ protein,Proteinvetenskap},
  language     = {eng},
  note         = {Student Paper},
  title        = {Purification and crystallization of the I subunit of magnesium chelatase},
  year         = {2011},
}