Purification and crystallization of the I subunit of magnesium chelatase
(2011) KEMT10 20111Department of Chemistry
- Abstract
- The I subunit of magnesium chelatase is an AAA+ protein which binds and
hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/3053630
- author
- Lv, You LU
- supervisor
- organization
- course
- KEMT10 20111
- year
- 2011
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Magnesium chelatase, BchI, ChlI, AAA+ protein, Proteinvetenskap
- language
- English
- id
- 3053630
- date added to LUP
- 2012-09-19 11:48:11
- date last changed
- 2012-09-19 11:48:11
@misc{3053630, abstract = {{The I subunit of magnesium chelatase is an AAA+ protein which binds and hydrolyzes ATP to drive the insertion of Mg2+ into protoporphyrin IX during bio-synthesis of bacteriochlorophyll and chlorophyll. The purification method and co-crystallization conditions of the Rhodobacter capsulatus BchI (bacteriochlorophyll Magnesium Chelatase I subunit) protein with either ADP or AMPPNP and crystallization conditions of Synechocystissp sp 6803 ChlI (chlorophyll Magnesium Chelatase I subunit) protein are described. This is the first report of a successful preparation of the crystals of BchI-ADP complex, the crystals of BchI-AMPPNP complex and crystals of ChlI protein. The crystals of both proteins will be used for further structure determination.}}, author = {{Lv, You}}, language = {{eng}}, note = {{Student Paper}}, title = {{Purification and crystallization of the I subunit of magnesium chelatase}}, year = {{2011}}, }