Identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa
(2001) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 109(7-8). p.551-558- Abstract
- The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates,... (More)
- The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates, identified as Staphylococcus aureus, Proteus vulgaris and Bacteroides merdae, were not found as a dominating part of the mucosal flora. Treatment with L. plantarum before liver injury decreased translocation and made the intestinal flora increasingly dominated by lactobacilli. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1122498
- author
- Wang, Mei LU ; Adawi, Diya LU ; Molin, Göran LU ; Pettersson, Bertil ; Jeppsson, Bengt LU and Ahrné, Siv LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacterial translocation, rat intestinal flora, probiotics, Lactobacillus plantarum, acute liver injury
- in
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- volume
- 109
- issue
- 7-8
- pages
- 551 - 558
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:11552954
- scopus:0034849841
- ISSN
- 1600-0463
- DOI
- 10.1111/j.1600-0463.2001.907810.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Surgery Research Unit (013242220), Food Technology (011001017), Emergency medicine/Medicine/Surgery (013240200), Applied Nutrition and Food Chemistry (011001300)
- id
- b0a57ea7-772c-4fa2-a606-18a4afe16bcb (old id 1122498)
- date added to LUP
- 2016-04-01 11:54:34
- date last changed
- 2023-11-11 05:38:46
@article{b0a57ea7-772c-4fa2-a606-18a4afe16bcb, abstract = {{The bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after D-galactosamine-induced liver injury, with and without pretreatment with Lactobacillus plantarum DSM 9843, were studied in the rat. Dominating representatives were identified to species level by 16S rDNA sequencing and typed by randomly amplified polymorphic DNA (RAPD) and by restriction endonuclease analysis (REA) for strain definition. It was proven that bacterial strains from the intestine occur at extraintestinal sites after liver injury. Lactobacillus spp. dominated the intestinal flora and were also the most frequently found genus in the liver and the mesenteric lymph nodes. Some of the blood isolates, identified as Staphylococcus aureus, Proteus vulgaris and Bacteroides merdae, were not found as a dominating part of the mucosal flora. Treatment with L. plantarum before liver injury decreased translocation and made the intestinal flora increasingly dominated by lactobacilli.}}, author = {{Wang, Mei and Adawi, Diya and Molin, Göran and Pettersson, Bertil and Jeppsson, Bengt and Ahrné, Siv}}, issn = {{1600-0463}}, keywords = {{Bacterial translocation; rat intestinal flora; probiotics; Lactobacillus plantarum; acute liver injury}}, language = {{eng}}, number = {{7-8}}, pages = {{551--558}}, publisher = {{John Wiley & Sons Inc.}}, series = {{APMIS : acta pathologica, microbiologica, et immunologica Scandinavica}}, title = {{Identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa}}, url = {{http://dx.doi.org/10.1111/j.1600-0463.2001.907810.x}}, doi = {{10.1111/j.1600-0463.2001.907810.x}}, volume = {{109}}, year = {{2001}}, }