Protein composition in different phases obtained by the ultracentrifugation of dough.
(2003) In Acta Agronomica Hungarica 51(2). p.163-172- Abstract
- Ultracentrifugation was used as a non-destructive method to separate dough into liquid, gel, gluten, starch and bottom phases. The protein composition in the different phases was investigated for dough prepared from spring wheat (Triticum aestivum L.). The SDS-PAGE, SE-HPLC and RP-HPLC methods were used for the analysis. The wheat protein composition of the liquid and gel phases consisted of albumins, globulins and traces of gliadins and glutenins. The gluten phase contained proteins extractable with all the extraction buffers used. A similar protein composition was found in the starch and bottom phases, but in considerably lower amounts. Specific LMW glutenin subunits were identified in the gluten phase by RP-HPLC. The albumin composition... (More)
- Ultracentrifugation was used as a non-destructive method to separate dough into liquid, gel, gluten, starch and bottom phases. The protein composition in the different phases was investigated for dough prepared from spring wheat (Triticum aestivum L.). The SDS-PAGE, SE-HPLC and RP-HPLC methods were used for the analysis. The wheat protein composition of the liquid and gel phases consisted of albumins, globulins and traces of gliadins and glutenins. The gluten phase contained proteins extractable with all the extraction buffers used. A similar protein composition was found in the starch and bottom phases, but in considerably lower amounts. Specific LMW glutenin subunits were identified in the gluten phase by RP-HPLC. The albumin composition differed in the gel phase compared to the gluten and bottom phases. Differences in protein composition due to mixing methods were detected only for the albumin composition in the liquid phases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128271
- author
- Kukhaite, R ; Larsson, Helena LU and Johansson, E
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Agronomica Hungarica
- volume
- 51
- issue
- 2
- pages
- 163 - 172
- publisher
- Akademiai Kiado
- external identifiers
-
- scopus:3042840169
- ISSN
- 0238-0161
- DOI
- 10.1556/AAgr.51.2003.2.4
- language
- English
- LU publication?
- yes
- id
- 93eac756-2ff2-4bcd-8b35-0034986cc945 (old id 128271)
- date added to LUP
- 2016-04-01 16:59:53
- date last changed
- 2023-09-05 05:15:12
@article{93eac756-2ff2-4bcd-8b35-0034986cc945,
abstract = {{Ultracentrifugation was used as a non-destructive method to separate dough into liquid, gel, gluten, starch and bottom phases. The protein composition in the different phases was investigated for dough prepared from spring wheat (Triticum aestivum L.). The SDS-PAGE, SE-HPLC and RP-HPLC methods were used for the analysis. The wheat protein composition of the liquid and gel phases consisted of albumins, globulins and traces of gliadins and glutenins. The gluten phase contained proteins extractable with all the extraction buffers used. A similar protein composition was found in the starch and bottom phases, but in considerably lower amounts. Specific LMW glutenin subunits were identified in the gluten phase by RP-HPLC. The albumin composition differed in the gel phase compared to the gluten and bottom phases. Differences in protein composition due to mixing methods were detected only for the albumin composition in the liquid phases.}},
author = {{Kukhaite, R and Larsson, Helena and Johansson, E}},
issn = {{0238-0161}},
language = {{eng}},
number = {{2}},
pages = {{163--172}},
publisher = {{Akademiai Kiado}},
series = {{Acta Agronomica Hungarica}},
title = {{Protein composition in different phases obtained by the ultracentrifugation of dough.}},
url = {{http://dx.doi.org/10.1556/AAgr.51.2003.2.4}},
doi = {{10.1556/AAgr.51.2003.2.4}},
volume = {{51}},
year = {{2003}},
}