Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells
Li, Shaoshan ; Paulsson, Markus LU and Björn, Lars Olof LU
(2002)
In Journal of Photochemistry and Photobiology, B: Biology
66(1).
p.67-72
- Abstract
- Two photoproducts of DNA damage, i.e. cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs), induced by UV-B radiation in suspension-cultured tobacco cells were quantified by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. CPDs and 6-4PPs were induced in tobacco cells by UV-B radiation. Photorepair of CPDs was faster than that of 6-4PPs. UV-B radiation induces formation of CPDs and 6-4PPs even at 0 °C, but low temperature significantly decreases the UV-B-induced (in contrast to UV-C-induced) formation of CPDs and 6-4PPs. Low temperature also retarded the removal of CPDs and 6-4PPs under white light, and almost no photorepair of CPDs and 6-4PPs was detected at 0 °C. When purified DNA from tobacco cells... (More)
- Two photoproducts of DNA damage, i.e. cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs), induced by UV-B radiation in suspension-cultured tobacco cells were quantified by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. CPDs and 6-4PPs were induced in tobacco cells by UV-B radiation. Photorepair of CPDs was faster than that of 6-4PPs. UV-B radiation induces formation of CPDs and 6-4PPs even at 0 °C, but low temperature significantly decreases the UV-B-induced (in contrast to UV-C-induced) formation of CPDs and 6-4PPs. Low temperature also retarded the removal of CPDs and 6-4PPs under white light, and almost no photorepair of CPDs and 6-4PPs was detected at 0 °C. When purified DNA from tobacco cells grown in darkness was irradiated with UV-B, formation of CPDs and 6-4PPs took place at the same speed at different temperatures. It indicated that formation of CPDs and 6-4PPs induced by UV-B was temperature-independent in a non-cellular system. Based on our results for suspension-cultured tobacco cells, not only the photorepair but also UV-B-induced formation of CPDs and 6-4PPs are temperature-dependent.
This is the final, accepted and revised manuscript of this article. Use alternative location to go to the published article. Requires subscription. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/133537
- author
- Li, Shaoshan
; Paulsson, Markus
LU
and Björn, Lars Olof
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Photochemistry and Photobiology, B: Biology
- volume
- 66
- issue
- 1
- pages
- 67 - 72
- publisher
- Elsevier
- external identifiers
-
- wos:000174008300010
- pmid:11849985
- scopus:0036471457
- ISSN
- 1011-1344
- DOI
- 10.1016/S1011-1344(01)00277-9
- project
- Photobiology
- language
- English
- LU publication?
- yes
- id
- da8000d1-ddab-46a7-acf3-ad353780b5cf (old id 133537)
- date added to LUP
- 2016-04-01 15:59:02
- date last changed
- 2022-02-20 02:29:25
@article{da8000d1-ddab-46a7-acf3-ad353780b5cf,
abstract = {{Two photoproducts of DNA damage, i.e. cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs), induced by UV-B radiation in suspension-cultured tobacco cells were quantified by enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies. CPDs and 6-4PPs were induced in tobacco cells by UV-B radiation. Photorepair of CPDs was faster than that of 6-4PPs. UV-B radiation induces formation of CPDs and 6-4PPs even at 0 °C, but low temperature significantly decreases the UV-B-induced (in contrast to UV-C-induced) formation of CPDs and 6-4PPs. Low temperature also retarded the removal of CPDs and 6-4PPs under white light, and almost no photorepair of CPDs and 6-4PPs was detected at 0 °C. When purified DNA from tobacco cells grown in darkness was irradiated with UV-B, formation of CPDs and 6-4PPs took place at the same speed at different temperatures. It indicated that formation of CPDs and 6-4PPs induced by UV-B was temperature-independent in a non-cellular system. Based on our results for suspension-cultured tobacco cells, not only the photorepair but also UV-B-induced formation of CPDs and 6-4PPs are temperature-dependent.<br/><br>
This is the final, accepted and revised manuscript of this article. Use alternative location to go to the published article. Requires subscription.}},
author = {{Li, Shaoshan and Paulsson, Markus and Björn, Lars Olof}},
issn = {{1011-1344}},
language = {{eng}},
number = {{1}},
pages = {{67--72}},
publisher = {{Elsevier}},
series = {{Journal of Photochemistry and Photobiology, B: Biology}},
title = {{Temperature-dependent formation and photorepair of DNA damage induced by UV-B radiation in suspension-cultured tobacco cells}},
url = {{https://lup.lub.lu.se/search/files/4534196/624379.pdf}},
doi = {{10.1016/S1011-1344(01)00277-9}},
volume = {{66}},
year = {{2002}},
}