Analysis of chlormequat in human urine as a biomarker of exposure using liquid chromatography triple quadrupole mass spectrometry.
(2011) In Journal of Chromatography. B 879(19). p.1551-1556- Abstract
- In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human... (More)
- In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human experimental oral exposure to CCC. Two healthy volunteers received 25μg/kg b.w. CCC in a single oral dose followed by urine sampling for 46h post-exposure. The CCC was estimated to follow a first order kinetic and a two compartment model with an elimination half-life of 2-3h and 10-14h respectively. One hundred 24h urine samples were collected from non-occupationally exposed individuals in the general population in southern Sweden. All samples had detectable levels above the LOD 0.1ng/mL urine. The median levels were 4ng/mL of CCC in unadjusted urine. The levels found in the population samples are several magnitudes lower than those found in the experimental exposure, which corresponds to an oral exposure of 50% of the ADI for CCC. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1937050
- author
- Lindh, Christian LU ; Littorin, Margareta LU ; Johannesson, Gunvor and Jönsson, Bo A LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography. B
- volume
- 879
- issue
- 19
- pages
- 1551 - 1556
- publisher
- Elsevier
- external identifiers
-
- wos:000291330600009
- pmid:21497564
- scopus:79955896812
- pmid:21497564
- ISSN
- 1873-376X
- DOI
- 10.1016/j.jchromb.2011.03.046
- language
- English
- LU publication?
- yes
- id
- 26b1e8c3-4baf-4d65-9260-a29ffedc62db (old id 1937050)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21497564?dopt=Abstract
- date added to LUP
- 2016-04-01 09:57:51
- date last changed
- 2022-04-12 00:41:25
@article{26b1e8c3-4baf-4d65-9260-a29ffedc62db, abstract = {{In this study, a method using liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) is described for the analysis of the plant growth regulator chlormequat (CCC) in human urine. Analysis was carried out using selected reaction monitoring (SRM) in the positive ion mode. [(2)H(4)] labeled CCC as internal standard (IS) was used for quantification of CCC. The limit of detection (LOD) was determined to 0.1ng/mL. The method was linear in the range 0.3-800ng/mL urine and had a within-run precision of 4-9%. The between-run precision was determined at urine levels of 7.0 and 31ng/mL and found to be 5 and 6% respectively. The reproducibility was 3-6%. To validate CCC as a biomarker of exposure, the method was applied in a human experimental oral exposure to CCC. Two healthy volunteers received 25μg/kg b.w. CCC in a single oral dose followed by urine sampling for 46h post-exposure. The CCC was estimated to follow a first order kinetic and a two compartment model with an elimination half-life of 2-3h and 10-14h respectively. One hundred 24h urine samples were collected from non-occupationally exposed individuals in the general population in southern Sweden. All samples had detectable levels above the LOD 0.1ng/mL urine. The median levels were 4ng/mL of CCC in unadjusted urine. The levels found in the population samples are several magnitudes lower than those found in the experimental exposure, which corresponds to an oral exposure of 50% of the ADI for CCC.}}, author = {{Lindh, Christian and Littorin, Margareta and Johannesson, Gunvor and Jönsson, Bo A}}, issn = {{1873-376X}}, language = {{eng}}, number = {{19}}, pages = {{1551--1556}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Analysis of chlormequat in human urine as a biomarker of exposure using liquid chromatography triple quadrupole mass spectrometry.}}, url = {{https://lup.lub.lu.se/search/files/1427400/1971357.pdf}}, doi = {{10.1016/j.jchromb.2011.03.046}}, volume = {{879}}, year = {{2011}}, }